Regional Ocular Inflammatory Service, Bristol Eye Hospital, Bristol BS1 2LX, UK.
Mediators Inflamm. 2012;2012:863418. doi: 10.1155/2012/863418. Epub 2012 Oct 24.
A correct diagnosis of uveitis is often challenging, given the wide range of possible underlying conditions and the lack of typical phenotypes. Management decisions may be difficult in view of the risk of visual loss with either inappropriate or delayed therapy. Analysis of the vitreous may therefore be used to provide the clinician with valuable information. In this paper, we describe the main clinical situations in which vitreous sampling is indicated and provide some guidance to clinicians for tailoring their requests. These situations include suspected intraocular infection and suspected intraocular malignancy. We describe the principal tests carried out on vitreous samples, including cultures, polymerase chain reaction-based testing, and cytokine analysis. Limitations of the tests used are likely to become less as more advanced testing methods are introduced. The importance of selecting the appropriate investigations to support a clinical suspicion is emphasised, as is the interpretation of test results within a clinical context.
葡萄膜炎的正确诊断往往具有挑战性，因为可能存在多种潜在情况，且缺乏典型的表型。鉴于不适当或延迟治疗可能导致视力丧失，管理决策可能较为困难。因此，对玻璃体进行分析可能有助于为临床医生提供有价值的信息。在本文中，我们描述了在哪些主要临床情况下需要进行玻璃体取样，并为临床医生提供了一些指导，以便根据他们的需求进行定制。这些情况包括疑似眼内感染和疑似眼内恶性肿瘤。我们描述了在玻璃体样本上进行的主要测试，包括培养、聚合酶链反应检测和细胞因子分析。随着更先进的检测方法的引入，使用的检测方法的局限性可能会越来越小。我们强调了选择适当的检查来支持临床怀疑的重要性，以及在临床背景下解释检查结果的重要性。
