Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Nationalestraat 155, 2000 Antwerpen, Belgium.
Expert Rev Mol Diagn. 2012 Sep;12(7):719-30. doi: 10.1586/erm.12.72.
This article will review the most recent progress in the molecular diagnosis of sleeping sickness and its potential role in patient management and disease control. While PCR remains restricted to research and reference laboratories, promising alternative molecular platforms have emerged over the last few years. Several loop-mediated isothermal amplification assays have been developed for detection and identification of the parasite with reported high analytical sensitivity and specificity. Simplified loop-mediated isothermal amplification formats have been designed and are undergoing evaluation studies in the field. Accurate diagnosis based on specific detection of the parasite's ribosomal RNA has been made possible by the isothermal nucleic acid sequence-based amplification and by direct hybridization with fluorescent detection probes. In addition to the technological progress, the authors also discuss the diagnostic performance of molecular tests in the most recent clinical evaluation studies and briefly present some viewpoints for the near future.
本文将综述昏睡病分子诊断的最新进展及其在患者管理和疾病控制方面的潜在作用。虽然聚合酶链反应（PCR）仍然局限于研究和参考实验室，但在过去几年中出现了一些有前途的替代分子平台。已经开发了几种环介导等温扩增检测方法来检测和鉴定寄生虫，报道称这些方法具有高分析灵敏度和特异性。简化的环介导等温扩增格式已被设计并正在现场进行评估研究。基于寄生虫核糖体 RNA 的特异性检测使得等温核酸序列扩增和直接与荧光检测探针杂交成为可能。除了技术进展，作者还讨论了分子检测在最近的临床评估研究中的诊断性能，并简要介绍了一些近期的观点。
