Ngotho Maina, Kagira John Maina, Gachie Beatrice Muthoni, Karanja Simon Muturi, Waema Maxwell Wambua, Maranga Dawn Nyawira, Maina Naomi Wangari
Animal Science Department, Institute of Primate Research (IPR), P.O. Box 24481, Karen, Nairobi 00502, Kenya.
Animal Health and Production Department, College of Agriculture and Natural Resources, Jomo Kenyatta University of Agriculture and Technology (JKUAT), P.O. Box 62000, Nairobi 00200, Kenya.
Biomed Res Int. 2015;2015:867846. doi: 10.1155/2015/867846. Epub 2015 Oct 4.
Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T. b. gambiense infection in a vervet monkey HAT model. Six vervet monkeys were experimentally infected with T. b. gambiense IL3253 and monitored for 180 days after infection. Parasitaemia was scored daily. Blood, cerebrospinal fluid (CSF), saliva, and urine samples were collected weekly. PCR and LAMP were performed on serum, CSF, saliva, and urine samples. The detection by LAMP was significantly higher than that of parasitological methods and PCR in all the samples. The performance of LAMP varied between the samples and was better in serum followed by saliva and then urine samples. In the saliva samples, LAMP had 100% detection between 21 and 77 dpi, whereas in urine the detection it was slightly lower, but there was over 80% detection between 28 and 91 dpi. However, LAMP could not detect trypanosomes in either saliva or urine after 140 and 126 dpi, respectively. The findings of this study emphasize the importance of LAMP in diagnosis of HAT using saliva and urine samples.
人类非洲锥虫病（HAT）是一种由媒介传播的寄生性人畜共患病。由布氏冈比亚锥虫引起的这种疾病在非洲最为普遍。由于缺乏灵敏的诊断技术，早期诊断受到阻碍。本研究探讨了环介导等温扩增技术（LAMP）和聚合酶链反应（PCR）在检测黑长尾猴HAT模型中布氏冈比亚锥虫感染方面的潜力。六只黑长尾猴经实验感染布氏冈比亚锥虫IL3253，并在感染后监测180天。每天对虫血症进行评分。每周采集血液、脑脊液（CSF）、唾液和尿液样本。对血清、CSF、唾液和尿液样本进行PCR和LAMP检测。在所有样本中，LAMP检测的阳性率显著高于寄生虫学方法和PCR。LAMP在不同样本中的表现有所不同，在血清中表现更好，其次是唾液，然后是尿液样本。在唾液样本中，LAMP在感染后21至77天的检测阳性率为100%，而在尿液中检测阳性率略低，但在感染后28至91天的检测阳性率超过80%。然而，LAMP分别在感染后140天和126天后无法在唾液或尿液中检测到锥虫。本研究结果强调了LAMP在使用唾液和尿液样本诊断HAT中的重要性。
