肥胖、糖尿病和高血压对肥胖女性网膜脂肪组织基因表达影响的相关性研究。

Correlative studies on the effects of obesity, diabetes and hypertension on gene expression in omental adipose tissue of obese women.

机构信息

Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, USA.

出版信息

Nutr Diabetes. 2011 Sep 26;1(9):e17. doi: 10.1038/nutd.2011.14.

DOI:10.1038/nutd.2011.14

PMID:23154658

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3302166/

Abstract

OBJECTIVE

A major consequence of obesity is the enormous expansion of and enhanced inflammatory response seen in visceral adipose tissue. I hypothesized that the expression of inflammatory markers in visceral omental fat would correlate with the extent of visceral adiposity as measured by waist circumference or body mass index and that diabetes and hypertension, defined as subjects taking anti-hypertensive drugs, would be associated with changes in mRNA expression in visceral fat.

DESIGN AND METHODS

The expression of 106 mRNAs by RT-PCR was examined in observational studies using extracts of omental fat of obese women undergoing bariatric surgery as well as the circulating levels of some adipokines. We also compared the mRNA levels of 65 proteins in omental fat removed during gastric bypass surgery of women with and without hypertension and those with type 2 diabetes.

RESULTS

Out of 106 mRNAs the expression of 10 mRNAs in omental fat of women not taking anti-hypertensive drugs correlated with waist circumference while 7 different mRNAs had significant correlations with circulating glucose. The correlations of waist circumference with mRNA expression were abolished, except for interleukin (IL)-1 receptor antagonist (IL-1RA), in women taking anti-hypertensive drugs. The correlations of blood glucose with omental fat mRNA expression were abolished, except for that of Akt1 and Akt2, in women taking anti-hypertensive drugs. However, the expression of 4 different mRNAs in omental fat was affected by circulating glucose in subjects taking anti-hypertensive drugs. The circulating levels of IL-1 RA, but not fatty acid binding protein 4, adipsin and phospholipase A2, correlated with both waist circumference and mRNA expression in omental fat.

CONCLUSION

In female bariatric surgery patients, the mRNA expression of some proteins in omental fat was affected by the degree of obesity, whereas hypertension and diabetes affected a separate set of mRNAs.Nutrition and Diabetes (2011) 1, e17; doi:10.1038/nutd.2011.14; published online 26 September 2011.

摘要

目的

肥胖的一个主要后果是内脏脂肪组织的巨大扩张和增强的炎症反应。我假设内脏网膜脂肪中炎症标志物的表达与腰围或体重指数所衡量的内脏肥胖程度相关，并且糖尿病和高血压（定义为服用抗高血压药物的患者）与内脏脂肪中 mRNA 表达的变化相关。

设计和方法

使用接受减肥手术的肥胖女性的网膜脂肪提取物，在观察性研究中通过 RT-PCR 检查 106 个 mRNA 的表达，以及一些脂肪因子的循环水平。我们还比较了患有高血压和 2 型糖尿病的女性在胃旁路手术中去除的网膜脂肪中 65 种蛋白质的 mRNA 水平。

结果

在未服用抗高血压药物的女性的网膜脂肪中，106 个 mRNA 中有 10 个的表达与腰围相关，而 7 个不同的 mRNA 与循环葡萄糖有显著相关性。在服用抗高血压药物的女性中，除白细胞介素 1 受体拮抗剂（IL-1RA）外，腰围与 mRNA 表达的相关性被消除。在服用抗高血压药物的女性中，除 Akt1 和 Akt2 外，血糖与网膜脂肪 mRNA 表达的相关性被消除。然而，在服用抗高血压药物的受试者中，4 种不同的 mRNA 在网膜脂肪中的表达受循环葡萄糖的影响。白细胞介素 1 RA 的循环水平，但不是脂肪酸结合蛋白 4、 adiposin 和磷脂酶 A2，与腰围和网膜脂肪中的 mRNA 表达都相关。