Kulkarni Roshan R, Shurpali Ketaki, Gawde Rupesh L, Sarkar Dhiman, Puranik Vedavati G, Joshi Swati P
Divison of Organic Chemistry, National Chemical Laboratory, Pune 411008, India.
J Asian Nat Prod Res. 2012;14(12):1162-8. doi: 10.1080/10286020.2012.738672. Epub 2012 Nov 19.
New phyllocladane diterpene, phyllocladan-16α,17-dihydroxy-19-oic acid (1), together with known phyllocladane diterpene, phyllocladan-16α,19-diol (2), cembrane diterpene ovatodiolide (3), sitosteryl-3-O-β-D-glucoside (4), and verbascoside (5), were isolated from aerial parts of Anisomeles heyneana. The structure of compound 1 was elucidated by 1D and 2D NMR analyses which included HSQC, HMBC, and nuclear overhauser effect spectroscopy (NOESY) experiments as well as X-ray crystallography. This is the first report of phyllocladane diterpenes from genus Anisomeles. Compounds 1, 3, 4, and 5 were evaluated for inhibition of Mycobacterium tuberculosis and 3 was found to exhibit anti-mycobacterial activity with IC₉₀ 6.53 μg/ml. Compounds 1, 3, and 5, at 100 μg/ml, were also evaluated for inhibition of Thp-1 cell lines, and compounds 1 and 3 showed 59.02% and 96.4% inhibitions, respectively.
从毛罗勒（Anisomeles heyneana）地上部分分离得到新的叶状枝烷二萜——叶状枝烷 - 16α,17 - 二羟基 - 19 - 酸（1），以及已知的叶状枝烷二萜——叶状枝烷 - 16α,19 - 二醇（2）、西松烯二萜卵叶二内酯（3）、谷甾醇 - 3 - O - β - D - 葡萄糖苷（4）和毛蕊花糖苷（5）。通过一维和二维核磁共振分析（包括HSQC、HMBC和核Overhauser效应光谱（NOESY）实验）以及X射线晶体学阐明了化合物1的结构。这是首次从毛罗勒属植物中报道叶状枝烷二萜。对化合物1、3、4和5进行了抗结核分枝杆菌活性评估，发现化合物3具有抗分枝杆菌活性，IC₉₀为6.53μg/ml。还对化合物1、3和5在100μg/ml浓度下抑制Thp - 1细胞系的活性进行了评估，化合物1和3的抑制率分别为59.02%和96.4%。
