Postgraduate Program of Dental College, Pontifical Catholic University of Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil.
J Oral Pathol Med. 2013 Mar;42(3):235-42. doi: 10.1111/jop.12018. Epub 2012 Nov 17.
This study aimed to analyze the oral lesions of chronic paracoccidioidomycosis concerning their histomorphometric, immunohistochemical, and clinical features in a standardized sample.
Fifty biopsy specimens of oral lesions of chronic paracoccidioidomycosis were submitted to hematoxylin and eosin (H&E), Grocott-Gomori and immunohistochemical staining. Data regarding disease duration and size and number of oral lesions, as well as erythrocytes, leukocytes, lymphocytes, hematocrit, hemoglobin, and erythrocyte sedimentation rate, were collected from medical charts. Granuloma density and number and diameter of buds and fungal cells, and IL-2, TNF-alpha and IFN-gamma expression, as well as clinical and hematological features, were quantified and correlated.
Bud diameter was significantly greater in intermediate density granulomas compared to higher density granulomas. The other variables (number of buds, number and diameter of fungi, expression of IL-2, TNF-alpha and IFN-gamma, and clinical and hematological features) did not significantly change with the density of granulomas. There was a positive correlation between bud number and fungal cell number (r = 0.834), bud diameter and fungal cell diameter (r = 0.496), erythrocytes and number of fungi (r = 0.420), erythrocytes and bud number (r = 0.408), and leukocytes and bud number (r = 0.396). Negative correlation occurred between number and diameter of fungi (r = -0.419), bud diameter and granuloma density (r = -0.367), TNF-alpha expression and number of fungi (r = -0.372), and TNF-alpha expression and bud number (r = -0.300).
The histological, immunological, and clinical features of oral lesions evaluated did not differ significantly between patients in our sample of chronic paracoccidioidomycosis. TNF-alpha levels were inversely correlated with intensity of infection.
本研究旨在分析慢性副球孢子菌病的口腔病变，涉及它们在标准化样本中的组织形态计量学、免疫组织化学和临床特征。
50 例慢性副球孢子菌病口腔病变的活检标本进行苏木精和伊红（H&E）、Grocott-Gomori 和免疫组织化学染色。从病历中收集有关疾病持续时间以及口腔病变的大小和数量，以及红细胞、白细胞、淋巴细胞、血细胞比容、血红蛋白和红细胞沉降率的数据。定量和相关性分析肉芽肿密度、芽和真菌细胞的数量和直径以及 IL-2、TNF-α 和 IFN-γ的表达，以及临床和血液学特征。
中间密度肉芽肿的芽直径明显大于高密度肉芽肿。其他变量（芽的数量、真菌的数量和直径、IL-2、TNF-α 和 IFN-γ的表达以及临床和血液学特征）与肉芽肿的密度没有显著变化。芽的数量与真菌细胞数量之间存在正相关（r = 0.834），芽直径与真菌细胞直径之间存在正相关（r = 0.496），红细胞与真菌数量之间存在正相关（r = 0.420），红细胞与芽数量之间存在正相关（r = 0.408），白细胞与芽数量之间存在正相关（r = 0.396）。真菌数量和直径之间存在负相关（r = -0.419），芽直径与肉芽肿密度之间存在负相关（r = -0.367），TNF-α 表达与真菌数量之间存在负相关（r = -0.372），TNF-α 表达与芽数量之间存在负相关（r = -0.300）。
在我们的慢性副球孢子菌病样本中，评估的口腔病变的组织学、免疫学和临床特征在患者之间没有显著差异。TNF-α 水平与感染强度呈负相关。
