Zhou Chen-fei, Ji Jun, Yuan Fei, Yu Ying-yan, Liu Bing-ya, Zhang Jun, Zhu Zheng-gang
Department of Surgery, Rui Jin Hospital, Shanghai Institute of Digestive Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Zhonghua Zhong Liu Za Zhi. 2012 Aug;34(8):592-5. doi: 10.3760/cma.j.issn.0253-3766.2012.08.007.
To determine the expression patterns of metastasis associated 1 family member 2 (MTA2) in gastric cancer and non-cancerous gastric mucosa, and analyze its relationship with nuclear transcription factor Sp1 expression.
Tissue samples and clinicopathological information from 83 gastric cancer patients, who underwent surgery, were collected from Shanghai Rui Jin Hospital. All samples included cancer tissue and non-cancerous mucosa which was 5 cm away from the tumor lesion. The expression of MTA2 and Sp1 were detected by immunohistochemistry (IHC) staining. The mRNA of MTA2 was also detected by reverse transcription-polymerase chain reaction (RT-PCR). SPSS software was used for statistical analysis.
The expression of MTA2 protein was significantly higher in primary lesions of the gastric cancer than that in non-cancerous mucosa by IHC (31.3% vs 12.0%, P < 0.01). MTA2 expression was closely related with tumor invasion or T staging (χ(2) = 5.677, P < 0.05). Yet, no significant relationship was observed between MTA2 expression and other clinicopathological parameters, including the age, sex, tumor differentiation, Lauren classification, lymph node metastasis, distant metastasis, as well as pathological staging. Furthermore, MTA2 expression was concomitant with Sp1 expression (r = 0.320, P < 0.05). Elevated MTA2 expression was observed in Sp1 positive cancer tissues (χ(2) = 9.565, P < 0.01). RT-PCR results also demonstrated that MTA2 mRNA was also highly expressed in the tissue samples with Sp1 expression.
MTA2 is highly expressed in the primary lesions of gastric cancer than that in adjacent non-cancerous tissues, and is closely related with tumor invasion. MTA2 expression is elevated in Sp1 positive gastric cancer.
确定转移相关蛋白1家族成员2（MTA2）在胃癌及癌旁胃黏膜中的表达模式，并分析其与核转录因子Sp1表达的关系。
收集上海瑞金医院83例行手术治疗的胃癌患者的组织样本及临床病理资料。所有样本包括癌组织及距肿瘤病变5 cm的癌旁黏膜。采用免疫组织化学（IHC）染色检测MTA2和Sp1的表达。同时采用逆转录-聚合酶链反应（RT-PCR）检测MTA2的mRNA。应用SPSS软件进行统计分析。
IHC结果显示，胃癌原发灶中MTA2蛋白表达显著高于癌旁黏膜（31.3% vs 12.0%，P < 0.01）。MTA2表达与肿瘤侵袭或T分期密切相关（χ(2)=5.677，P < 0.05）。然而，未观察到MTA2表达与其他临床病理参数之间存在显著相关性，这些参数包括年龄、性别、肿瘤分化程度、Lauren分型、淋巴结转移、远处转移以及病理分期。此外，MTA2表达与Sp1表达呈正相关（r = 0.320，P < 0.05）。在Sp1阳性的癌组织中观察到MTA2表达升高（χ(2)=9.565，P < 0.01）。RT-PCR结果也表明，在有Sp1表达的组织样本中MTA2 mRNA也高表达。
MTA2在胃癌原发灶中的表达高于相邻癌旁组织，且与肿瘤侵袭密切相关。在Sp1阳性的胃癌中MTA2表达升高。
