来自普通拟杆菌的解旋酶相关结构域BVU_0683(627 - 691)的溶液核磁共振结构为蛋白质结构域家族PF03457提供了首个结构覆盖，并表明该结构域与DNA结合。
Solution NMR structure of the helicase associated domain BVU_0683(627-691) from Bacteroides vulgatus provides first structural coverage for protein domain family PF03457 and indicates domain binding to DNA.
作者信息
Mills Jeffrey L, Acton Thomas B, Xiao Rong, Everett John K, Montelione Gaetano T, Szyperski Thomas
机构信息
Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
出版信息
J Struct Funct Genomics. 2013 Mar;14(1):19-24. doi: 10.1007/s10969-012-9148-0. Epub 2012 Nov 16.
Abstract
A high-quality NMR structure of the helicase associated (HA) domain comprising residues 627-691 of the 753-residue protein BVU_0683 from Bacteroides vulgatus exhibits an all α-helical fold. The structure presented here is the first representative for the large protein domain family PF03457 (currently 742 members) of HA domains. Comparison with structurally similar proteins supports the hypothesis that HA domains bind to DNA and that binding specificity varies greatly within the family of HA domains constituting PF03457.
摘要
来自普通拟杆菌的753个氨基酸残基的蛋白质BVU_0683中包含627 - 691位残基的解旋酶相关（HA）结构域的高质量核磁共振结构呈现出全α螺旋折叠。此处展示的结构是HA结构域的大型蛋白质结构域家族PF03457（目前有742个成员）的首个代表性结构。与结构相似蛋白质的比较支持了以下假说：HA结构域与DNA结合，并且在构成PF03457的HA结构域家族中，结合特异性差异很大。
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