Institute for Environmental Sciences, University of Koblenz-Landau, Fortstrasse 7, 76829, Landau (Pfalz), Germany.
Environ Sci Pollut Res Int. 2013 Jun;20(6):3735-42. doi: 10.1007/s11356-012-1311-4. Epub 2012 Nov 17.
A few studies reporting the effects of tebufenozide, a non-steroidal ecdysone agonist that mimics natural moulting hormones in chironomids exist in the literature. However, nothing is known about its chronic or multigenerational effects on the reproduction of aquatic insects, although tebufenozide is present in aquatic ecosystems. Here, we investigated the chronic toxicity of tebufenozide in two successive generations of Chironomus riparius using nominal concentrations that ranged from 4 to 26.2 μg/L. We started the test from the first instar larvae in the parental (P) generation, quantifying life cycle parameters (emergence, sex ratio, development rate, fecundity and fertility) until the emergence in the subsequent F1 generation. Results showed a reduction in reproduction and a significant decrease in male developmental rate of midges for all treatments, in the F1 generation compared with the P generation (paired t test; p<0.001). Two-way analysis of variance revealed a significant exposure × generation effect on male fraction with male fraction increasing (P generation) or decreasing (F1 generation) with increasing exposure. These effects on C. riparius underline the importance of conducting long-term studies with environmentally relevant concentrations to investigate population-level endpoints for endocrine disrupting chemicals.
一些研究报告了噻嗪酮的作用，噻嗪酮是一种非甾体蜕皮激素激动剂，可模拟摇蚊体内的天然蜕皮激素。然而，尽管噻嗪酮存在于水生生态系统中，但关于它对水生昆虫繁殖的慢性或多代影响仍一无所知。在这里，我们使用 4 至 26.2μg/L 的名义浓度，在连续两代 Chironomus riparius 中研究了噻嗪酮的慢性毒性。我们从亲代（P）代的第一龄幼虫开始测试，量化了生命周期参数（出现、性别比例、发育速度、繁殖力和生育力），直到随后的 F1 代出现。结果表明，与 P 代相比，所有处理组在 F1 代中繁殖减少，并且雄虫发育速度显著降低（配对 t 检验；p<0.001）。双因素方差分析显示，暴露与代际之间对雄性部分有显著影响，随着暴露的增加，雄性部分增加（P 代）或减少（F1 代）。这些对 C. riparius 的影响强调了用具有环境相关性的浓度进行长期研究的重要性，以研究内分泌干扰化学物质的种群水平终点。
