Institute for Environmental Sciences, University of Koblenz-Landau, Landau (Pfalz), Germany.
Ecotoxicol Environ Saf. 2011 Jul;74(5):1203-9. doi: 10.1016/j.ecoenv.2011.02.013. Epub 2011 Mar 10.
Teflubenzuron, a chitin synthesis inhibitor was used in a two-generation test with Chironomus riparius (Insecta) to assess effects over a full life cycle from the first-instar larvae in the parental (P) generation until emergence in the subsequent F1 generation. Sediment spiked with teflubenzuron ranging from 10 to 390.6 μg/kg sediment dry weight (dw) was used. EC₅₀-values for fecundity and fertility were 112.7 and 74.5 μg/kg dw, respectively. Significant adverse effects were observed compared to the solvent control for emergence rate (P < 0.01) and also for developmental rate. No observed effect concentrations values were lower for emergence rate in the F1 generation (62.5 μg/kg dw) than in the P generation (100 μg/kg dw), demonstrating that the F1 generation was more affected than the P generation. Thus, this two-generation test may help detecting population level effects as an amendment to the risk assessment for chronic exposures to endocrine disrupting compounds.
特氟隆，一种几丁质合成抑制剂，在摇蚊（昆虫）的两代试验中使用，以评估从亲代（P）代的第一龄幼虫到随后的 F1 代成虫的整个生命周期内的影响。用特氟隆污染的沉积物浓度范围为 10 至 390.6μg/kg 沉积物干重（dw）。产卵量和生育率的 EC₅₀值分别为 112.7 和 74.5μg/kg dw。与溶剂对照相比，孵化率（P<0.01）和发育率均出现显著的不利影响。F1 代的无观察效应浓度值（NOEC）比 P 代的孵化率（62.5μg/kg dw）低（100μg/kg dw），表明 F1 代比 P 代受到的影响更大。因此，这项两代试验可能有助于检测种群水平的影响，作为对内分泌干扰物慢性暴露风险评估的补充。
