Yuan Zhao-kai, Wang Li-ping, Huang Xian-ping
Key Discipline of Chinese Medical Diagnostics, Hunan University of Traditional Chinese Medicine, Changsha.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Oct;32(10):1313-8.
To explore the function and target pathway of the correlated differential gene of coronary heart disease (CHD) of blood stasis syndrome (BSS).
Patients of the genealogical CHD of BSS (group A) and the genealogical CHD of non-BSS (group B), the genealogical non-CHD of BSS (group C), the genealogical healthy subjects (group D), the non-genealogical CHD of BSS (group E), the non-genealogical healthy subjects (group F) were recruited in this study. The differential gene expression spectrums were studied using gene chip technique. The molecular functions of differential genes were analyzed and illustrated by gene ontology (GO) analysis. The differential gene pathways were found out at BioCarta and KEGG. The meaningful target pathways were screened by hypergeometric distribution statistical method. The differential genes were verified using Real-time fluorescent quantitative PCR.
(1) By screening the gene chip data (with FC > or =3), we found the expressions of differential genes of CHD of BSS were mainly involved in chemokine, interleukin cytokine, alexin system, matrix metal proteinase system, fibroblastic growth factor, endothelial cell adhesion molecule, and so on. (2) By GO analysis of related differential genes (P < 0.05), we found the molecular functions of differential genes associated with CHD BSS. (3) By BioCarta and KEGG pathway analysis, we found the target pathways of the hereditary correlated differential genes of CHD BSS with significance were mainly involved in inflammation, plaque formation, endothelial injury, and so on. The results of Real-time fluorescent quantitative RT-PCR proved the accuracy of the gene chip.
The hereditary correlated differential genes of CHD BSS were closely associated with inflammation, plaque formation, and endothelial injury.
探讨冠心病血瘀证相关差异基因的功能及靶向通路。
本研究招募了冠心病血瘀证家系患者（A组）、非血瘀证冠心病家系患者（B组）、血瘀证非冠心病家系患者（C组）、健康家系受试者（D组）、非家系冠心病血瘀证患者（E组）、非家系健康受试者（F组）。采用基因芯片技术研究差异基因表达谱。通过基因本体论（GO）分析对差异基因的分子功能进行分析和阐述。在BioCarta和KEGG中找出差异基因通路。采用超几何分布统计方法筛选有意义的靶向通路。使用实时荧光定量PCR对差异基因进行验证。
（1）通过筛选基因芯片数据（FC≥3），发现冠心病血瘀证差异基因的表达主要涉及趋化因子、白细胞介素细胞因子、补体系统、基质金属蛋白酶系统、成纤维细胞生长因子、内皮细胞黏附分子等。（2）通过对相关差异基因进行GO分析（P<0.05），发现了与冠心病血瘀证相关的差异基因的分子功能。（3）通过BioCarta和KEGG通路分析，发现冠心病血瘀证遗传相关差异基因的有意义靶向通路主要涉及炎症、斑块形成、内皮损伤等。实时荧光定量RT-PCR结果证明了基因芯片的准确性。
冠心病血瘀证遗传相关差异基因与炎症、斑块形成及内皮损伤密切相关。
