Zimran A, Forman L, Suzuki T, Dale G L, Beutler E
Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Am J Hematol. 1990 Apr;33(4):249-54. doi: 10.1002/ajh.2830330407.
It is generally recognized that the activities of some of the red cell enzymes decline as the cell ages. However, there is still a controversy regarding the rate at which this aging occurs. In the present study we applied newly developed technology for the specific isolation of maturing reticulocytes/erythrocytes for a more comprehensive study of in vivo aging of red cell enzymes in rabbits. Anemia was induced by repeated phlebotomy, and reticulocyte-rich erythrocytes were labeled with N-hydroxy succinimido-biotin and then transfused into a normal rabbit. These biotinylated cells were isolated at various time points by their affinity for an avidin support, and the enzymatic activity of 19 red cell enzymes was measured. We observed a biphasic pattern of decay for the activity of six age-dependent enzymes--aldolase, glutamate-oxaloacetate transaminase, glucose 6-phosphate dehydrogenase, hexokinase, pyrimidine 5'-nucleotidase, and pyruvate kinase.
人们普遍认为，随着红细胞老化，一些红细胞酶的活性会下降。然而，关于这种老化发生的速率仍存在争议。在本研究中，我们应用新开发的技术特异性分离成熟网织红细胞/红细胞，以更全面地研究兔体内红细胞酶的老化。通过反复放血诱导贫血，用N-羟基琥珀酰亚胺生物素标记富含网织红细胞的红细胞，然后输给正常兔。这些生物素化的细胞在不同时间点通过它们对抗生物素蛋白支持物的亲和力进行分离，并测定19种红细胞酶的酶活性。我们观察到六种年龄依赖性酶——醛缩酶、谷草转氨酶、葡萄糖6-磷酸脱氢酶、己糖激酶、嘧啶5'-核苷酸酶和丙酮酸激酶的活性呈现双相衰减模式。
