亚洲沙尘颗粒通过活性氧在支气管上皮细胞中诱导转化生长因子-β(1) 。
Asian Dust Particles Induce TGF-β(1) via Reactive Oxygen Species in Bronchial Epithelial Cells.
作者信息
Kyung Sun Young, Yoon Jin Young, Kim Yu Jin, Lee Sang Pyo, Park Jeong-Woong, Jeong Sung Hwan
机构信息
Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital, Gachon University of Medicine and Science, Incheon, Korea.
出版信息
Tuberc Respir Dis (Seoul). 2012 Aug;73(2):84-92. doi: 10.4046/trd.2012.73.2.84. Epub 2012 Aug 31.
BACKGROUND
Asian dust storms can be transported across eastern Asia. In vitro, Asian dust particle-induced inflammation and enhancement of the allergic reaction have been observed. However, the fibrotic effects of Asian dust particles are not clear. Production of transforming growth factor β(1) (TGF-β(1)) and fibronectin were investigated in the bronchial epithelial cells after exposure to Asian dust particulate matter (AD-PM10).
METHODS
During Asian dust storm periods, air samples were collected. The bronchial epithelial cells were exposed to AD-PM10 with and without the antioxidant, N-acetyl-L-cysteine (NAC). Then TGF-β(1) and fibronectin were detected by Western blotting. The reactive oxygen species (ROS) was detected by the measurement of dicholorodihydrofluorescin (DCF), using a FACScan, and visualized by a confocal microscopy.
RESULTS
The expression of TGF-β(1), fibronectin and ROS was high after being exposed to AD-PM10, compared to the control. NAC attenuated both TGF-β(1) and fibronectin expression in the AD-PM10-exposed the bronchial epithelial cells.
CONCLUSION
AD-PM10 may have fibrotic potential in the bronchial epithelial cells and the possible mechanism is AD-PM10-induced intracellular ROS.
背景
亚洲沙尘暴可跨越东亚地区传输。在体外实验中，已观察到亚洲沙尘颗粒可引发炎症并增强过敏反应。然而，亚洲沙尘颗粒的纤维化作用尚不清楚。本研究旨在探讨暴露于亚洲沙尘颗粒物（AD-PM10）后支气管上皮细胞中转化生长因子β(1)（TGF-β(1)）和纤连蛋白的产生情况。
方法
在亚洲沙尘暴期间采集空气样本。将支气管上皮细胞暴露于含有和不含有抗氧化剂N-乙酰-L-半胱氨酸（NAC）的AD-PM10中。然后通过蛋白质免疫印迹法检测TGF-β(1)和纤连蛋白。使用FACScan通过测量二氯二氢荧光素（DCF）来检测活性氧（ROS），并通过共聚焦显微镜进行可视化观察。
结果
与对照组相比，暴露于AD-PM10后，TGF-β(1)、纤连蛋白和ROS的表达均升高。NAC可减弱AD-PM10暴露的支气管上皮细胞中TGF-β(1)和纤连蛋白的表达。
结论
AD-PM10可能在支气管上皮细胞中具有纤维化潜能，其可能机制是AD-PM10诱导细胞内ROS产生。
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