Shrestha Raj, Nepal Ashwini Kumar, Lal Das Binod Kumar, Gelal Basanta, Lamsal Madhab
Department of Biochemistry, BP Koirala Institute of Health Sciences (BPKIHS), Nepal.
Asian Pac J Cancer Prev. 2012;13(9):4353-6. doi: 10.7314/apjcp.2012.13.9.4353.
Tobacco consumption is one of the leading causes of oral submucous fibrosis, oral cancer and even premature death. The present study was designed to compare the biochemical parameters and non- enzymatic antioxidant status and the lipid peroxidation products in pan masala tobacco users as compared with age-matched non-user controls.
Pan masala and tobacco users of age 33.2±9.94 years and age-matched controls (31.2±4.73 years) were enrolled for the study. Plasma levels of vitamin E, vitamin C, albumin, bilirubin, uric acid, glucose, urea, creatinine, aspartate amino transferase (AST), alanine amino transferase (ALT) were measured by standard methods. Serum malondialdehyde (MDA) levels were estimated as a measure of lipid peroxidation.
In the pan masala tobacco users, as compared to the controls, the level of vitamin C (68.5±5.9 vs 97.9±9.03 μmol/L, p≤0.05) vitamin E (18.4±5.3 vs 97.9±9.03 μmol/L, p≤0.001), albumin (37.5±7.01 vs 44.3±9.99 g/L, p≤0.001), and malondialdehyde (10.8±1.29 vs 1.72±1.15 nmol/ml, p≤0.001 ) were found to be significantly altered. Malondialdehyde was significantly correlated with vitamin E (r=1.00, p<0.001) and vitamin C (r =1.00, p<0.001) in pan masala tobacco users. Serum levels of AST (31.0±16.77 IU) and ALT (36.7±31.3 IU) in the pan masala tobacco users were significantly raised as compared to the controls (AST, 25.2±9.51 IU, p=0.038; ALT, 26.2±17.9 IU, p=0.038).
These findings suggest that pan masala tobacco users are in a state of oxidative stress promoting cellular damage. Non-enzymatic antioxidants are depleted in pan masala tobacco users with subsequent alteration in the biochemical parameters. Supplementation of antioxidants may prevent oxidative damage in pan masala tobacco users.
烟草消费是口腔黏膜下纤维化、口腔癌甚至过早死亡的主要原因之一。本研究旨在比较与年龄匹配的非使用者对照组相比，嚼烟使用者的生化参数、非酶抗氧化状态和脂质过氧化产物。
年龄在33.2±9.94岁的嚼烟和烟草使用者以及年龄匹配的对照组（31.2±4.73岁）被纳入研究。采用标准方法测量血浆中维生素E、维生素C、白蛋白、胆红素、尿酸、葡萄糖、尿素、肌酐、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）的水平。血清丙二醛（MDA）水平作为脂质过氧化的指标进行评估。
与对照组相比，嚼烟使用者中维生素C（68.5±5.9对97.9±9.03μmol/L，p≤0.05）、维生素E（18.4±5.3对97.9±9.03μmol/L，p≤0.001）、白蛋白（37.5±7.01对44.3±9.99g/L，p≤0.001）和丙二醛（10.8±1.29对1.72±1.15nmol/ml，p≤0.001）水平有显著变化。在嚼烟使用者中，丙二醛与维生素E（r = 1.00，p < 0.001）和维生素C（r = 1.00，p < 0.001）显著相关。与对照组相比，嚼烟使用者的血清AST（31.0±16.77IU）和ALT（36.7±31.3IU）水平显著升高（AST，25.2±9.51IU，p = 0.038；ALT，26.2±17.9IU，p = 0.038）。
这些发现表明嚼烟使用者处于促进细胞损伤的氧化应激状态。嚼烟使用者中非酶抗氧化剂减少，随后生化参数发生改变。补充抗氧化剂可能预防嚼烟使用者的氧化损伤。
