Lee Jin-A, Jung Bock-Gie, Lee Bong-Joo
Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Asian Pac J Cancer Prev. 2012;13(9):4655-60. doi: 10.7314/apjcp.2012.13.9.4655.
Opuntia humifusa, member of the Cactaceae family, was previously demonstrated to have radical scavenging, anti-inflammatory and anti-proliferative effects in in vitro models. It was suggested that O. humifusa could function in the prevention of carcinogenesis. To investigate the in vivo chemopreventive effect of O. humifusa, mice were fed a diet containing either 1% or 3% following 7, 12-dimethylbenz[a] anthracene (DMBA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) induction of skin carcinogenesis. Significant decrease in the numbers of papilloma and epidermal hyperplasia were observed in mice fed with O. humifusa, compared to the control group. O. humifusa also upregulated high total antioxidant capacity and level of phase II detoxifying enzyme such as superoxide dismutase and glutathione S-transferase activity in the skin. Lipid peroxidation activity level was measured in skin cytosol and significantly inhibited in 3% OH fed group compared to the control group. These results suggest that O. humifusa exerts chemopreventive effects on chemical carcinogenesis in mouse skin and that prevention effects are associated with reduction of oxidative stress via the modulation of cutaneous lipid peroxidation, enhancing of total antioxidant capacity especially in phase II detoxifying enzyme system and partial apoptotic influence.
仙人掌科植物平卧仙人掌（Opuntia humifusa）此前在体外模型中已被证明具有自由基清除、抗炎和抗增殖作用。有人认为平卧仙人掌可能在预防癌症发生中发挥作用。为了研究平卧仙人掌的体内化学预防作用，在给予7,12 - 二甲基苯并[a]蒽（DMBA）和12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）诱导皮肤癌发生后，给小鼠喂食含有1%或3%平卧仙人掌的饮食。与对照组相比，喂食平卧仙人掌的小鼠乳头状瘤数量和表皮增生明显减少。平卧仙人掌还上调了皮肤中的总抗氧化能力以及II期解毒酶如超氧化物歧化酶和谷胱甘肽S - 转移酶活性水平。在皮肤细胞溶质中测量脂质过氧化活性水平，与对照组相比，3%平卧仙人掌喂食组中该活性水平受到显著抑制。这些结果表明，平卧仙人掌对小鼠皮肤化学致癌具有化学预防作用，且预防作用与通过调节皮肤脂质过氧化、增强总抗氧化能力（特别是在II期解毒酶系统中）以及部分凋亡影响来降低氧化应激有关。
