Sugioka K, Nakano M
Biochim Biophys Acta. 1976 Feb 16;423(2):203-16. doi: 10.1016/0005-2728(76)90179-1.
A simplified system, consisting of NADPH, Fe3+-ADP, EDTA, liposomes, NADPH-cytochrome c reductase and Tris - HCl buffer (pH 6.8), has been employed in studies of the generation of singlet oxygen in NADPH-dependent microsomal lipid peroxidation. The light emitted by the system involves 1deltag type molecular oxygen identifiable by its characteristic emission spectrum and its behavior with beta-carotene. The generation of another excited species (a compound in the triplet state) could be demonstrated in this system by changes of light intensity and emission spectra which arise from photosensitizer (9,10-dibromoanthracene sulfonate, eosin, Rose-Bengal)-mediated energy transfers. Chemiluminescence in the visible region was markedly quenched by various radical trappers and by an inhibitor of NADPH-cytochrome c reductase, but not by superoxide dismutase. During the early stage of lipid peroxidation, the intensity of chemiluminescence was proportional to the square of the concentration of lipid peroxide. These characteristics suggest that singlet oxygen and a compound in the triplet state (probably a carbonyl compound) are generated by a self-reaction of lipid peroxy radicals.
一个简化系统，由还原型辅酶Ⅱ（NADPH）、三价铁 - 二磷酸腺苷（Fe3+-ADP）、乙二胺四乙酸（EDTA）、脂质体、NADPH - 细胞色素c还原酶和三羟甲基氨基甲烷 - 盐酸缓冲液（pH 6.8）组成，已被用于研究NADPH依赖性微粒体脂质过氧化过程中单线态氧的生成。该系统发出的光涉及通过其特征发射光谱及其与β - 胡萝卜素的反应可识别的1Δg型分子氧。通过光敏剂（9,10 - 二溴蒽磺酸盐、曙红、孟加拉玫瑰红）介导的能量转移引起的光强度和发射光谱变化，可以证明该系统中另一种激发态物种（三重态化合物）的生成。可见区域的化学发光被各种自由基捕获剂和NADPH - 细胞色素c还原酶抑制剂显著淬灭，但不被超氧化物歧化酶淬灭。在脂质过氧化的早期阶段，化学发光强度与脂质过氧化物浓度的平方成正比。这些特征表明单线态氧和三重态化合物（可能是羰基化合物）是由脂质过氧自由基的自反应产生的。
