开发和评估一种新型的半自动化艰难梭菌分型平台。
Development and evaluation of a novel, semiautomated Clostridium difficile typing platform.
机构信息
Department of Pathology and Immunology, Washington University School of Medicine in St Louis, St Louis, Missouri, USA.
出版信息
J Clin Microbiol. 2013 Feb;51(2):621-4. doi: 10.1128/JCM.02627-12. Epub 2012 Nov 21.
Abstract
We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsed-field gel electrophoresis.
摘要
我们描述了一种新颖的半自动化艰难梭菌分型平台，该平台基于 PCR-核糖体分型技术，并结合了半自动分子分型系统。该平台具有可重复性，且内部和外部试验变异性很小。该方法的分辨指数为 0.954，因此可与更繁琐的分型系统（如脉冲场凝胶电泳）相媲美。
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