Laboratoire de parasitologie-mycologie, plateau technique de biologie du CHU, 2, rue Angélique-Ducoudray, BP 37013, 21070 Dijon cedex, France.
J Mycol Med. 2012 Mar;22(1):14-20. doi: 10.1016/j.mycmed.2011.11.001. Epub 2011 Dec 28.
Study of the spatio-temporal fungal colonization in a new medical mycology laboratory.
A 17-month survey of airborne fungal contamination was conducted in a new medical mycology laboratory at a tertiary care university hospital. This survey was implemented at three different periods: before the new premises were occupied (period A), during the move into the new laboratory (period B) and after resumption of the mycological activities in these new premises (period C).
During period A, the airborne fungal load ranged from 2.3 to 6 cfu/m(3). The most frequently recovered airborne fungi were Penicillium spp. (75 to 100%). During period B, a dramatic increase in Penicillium chrysogenum conidia was observed in the air of the new laboratory (40 to 160 cfu/m(3)). During period C, the fungal load ranged from 4.5 to 8.4 cfu/m(3). Penicillium was the most common genus identified in rooms of the laboratory where no filamentous fungi were handled, while Aspergillus was clearly the predominant genus (78%) in the room dedicated to the culture of filamentous fungi.
We suggest that the specific fungal ecology in air of the room dedicated to the culture of filamentous fungi is due to the handling of a large number of medical strains of A. fumigatus.
研究新医学真菌学实验室中的时空真菌定殖。
在一家三级保健大学医院的新医学真菌学实验室中，进行了为期 17 个月的空气真菌污染调查。该调查在三个不同时期进行：新实验室启用前（A 期）、搬进新实验室期间（B 期）和在这些新场所恢复真菌学活动后（C 期）。
在 A 期，空气真菌负荷范围为 2.3 至 6 cfu/m³。最常回收的空气真菌是青霉属（75%至 100%）。在 B 期，新实验室空气中的青霉素 chrysogenum 分生孢子急剧增加（40 至 160 cfu/m³）。在 C 期，真菌负荷范围为 4.5 至 8.4 cfu/m³。在未处理丝状真菌的实验室房间中，青霉属是最常见的鉴定属，而曲霉属（78%）在专门用于培养丝状真菌的房间中则是明显的优势属。
我们认为，专门用于培养丝状真菌的房间空气中特定的真菌生态学是由于大量处理烟曲霉的医学菌株所致。
