CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Spain.
Nanomedicine. 2013 Jul;9(5):646-56. doi: 10.1016/j.nano.2012.11.002. Epub 2012 Nov 22.
We explore the synergistic effect of photothermal therapy and gene therapy, simultaneously triggered by silica-gold nanoshells (NS) or hollow gold nanoparticles (HGNPs) in human HeLa cells following near-infrared (NIR) light irradiation. Thermal transfer from NS was higher than that displayed by HGNPs, owing to a differential interaction of the nanomaterial with the biological environment. Under sublethal photothermal conditions, NS and HGNPs effectively modulated the expression levels of a DsRed-monomer reporter gene controlled by the highly heat-inducible human HSP70B promoter, as a function of nanomaterial concentration and length of laser exposure. Hyperthermia treatments at doses that do not promote cell death generated a lethal outcome in HeLa cells harboring the fusogenic GALV-FMG transgene under the control of the HSP70B promoter. Combination of lethal photothermia with the triggering of the cytotoxic transgene resulted in a dramatic increase of the cell-ablation area as a result of the synergistic activity established.
In this study photothermal therapy and gene therapy, simultaneously triggered by silica-gold nanoshells or hollow gold nanoparticles, was investigated in human HeLa cells following near-infrared (NIR) light irradiation. It is shown that the combination of lethal photothermia with the triggering of the cytotoxic transgene at sublethal levels results in a synergistic cytotoxic effect in vitro.
本研究探讨了近红外（NIR）光照射下，硅-金纳米壳或中空金纳米颗粒同时引发的光热疗法和基因疗法的协同效应。在人宫颈癌细胞（HeLa）中进行了研究。由于纳米材料与生物环境的相互作用不同，金纳米壳的热传递高于中空金纳米颗粒。在亚致死光热条件下，纳米壳和中空金纳米颗粒有效调节了由高度热诱导的人 HSP70B 启动子控制的 DsRed-单体报告基因的表达水平，其功能取决于纳米材料的浓度和激光暴露时间。在不促进细胞死亡的剂量下进行的热疗会导致在 HSP70B 启动子控制下携带融合基因 GALV-FMG 的 HeLa 细胞致死。致死光热与细胞毒性转基因的触发相结合，由于协同作用的建立，导致细胞消融区域显著增加。
在这项研究中，研究了近红外（NIR）光照射下，硅-金纳米壳或中空金纳米颗粒同时引发的光热疗法和基因疗法。结果表明，在亚致死水平下，致死光热与细胞毒性转基因的触发相结合，可在体外产生协同的细胞毒性作用。
