评价使用血浆制备管进行 COBAS AmpliPrep/COBAS TaqMan HIV-1 版本 2.0 上的 HIV 病毒载量检测。
Evaluation of the use of plasma preparation tubes for HIV viral load testing on the COBAS AmpliPrep/COBAS TaqMan HIV-1 version 2.0.
机构信息
Department of Medical Microbiology and Virology, University of the Free State/National Health Laboratory Service, Bloemfontein, South Africa.
出版信息
J Virol Methods. 2013 Feb;187(2):248-50. doi: 10.1016/j.jviromet.2012.11.019. Epub 2012 Nov 23.
HIV viral load monitoring forms an essential part of the management of patients receiving antiretroviral therapy, but transport of samples without loss of RNA integrity may be problematic in resource limited settings. The use of plasma preparation tubes (PPT) which can be centrifuged to separate cellular components before transport may provide a simple and cost-effective alternative to standard EDTA samples. We investigated whether PPT generated reliable results using the COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HIV-1 test version 2.0 (CAP/CTM HIV-1 v2.0). The mean difference between EDTA and PPT prepared samples (n=261) was acceptable (log 0.04 copies/ml, percentage similarity CV 3.53%). PPT can be used for viral load testing on the CAP/CTM HIV-1 v2.0.
HIV 病毒载量监测是接受抗逆转录病毒治疗的患者管理的重要组成部分，但在资源有限的环境中，样本在运输过程中不失真可能是一个问题。使用血浆制备管（PPT）在运输前离心以分离细胞成分，可能是一种简单且具有成本效益的替代标准 EDTA 样本的方法。我们研究了使用 COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HIV-1 测试版本 2.0（CAP/CTM HIV-1 v2.0）时，PPT 是否能产生可靠的结果。EDTA 和 PPT 制备样本之间的平均差异（n=261）可接受（log 0.04 拷贝/ml，百分比相似性 CV 3.53%）。PPT 可用于 CAP/CTM HIV-1 v2.0 的病毒载量检测。
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