Graduate Program of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil.
Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Brazil.
Microb Pathog. 2019 Jul;132:137-140. doi: 10.1016/j.micpath.2019.04.036. Epub 2019 Apr 24.
The role of cholinesterase in inflammatory reactions has been described in several infectious diseases. However, in Brucella spp. this has not yet been studied. Therefore, the objective of this study was to evaluate whether experimental infection by Brucella ovis alters the cholinergic activity in pro- or anti-inflammatory responses to the disease. For the study 48 mice were used, 24 infected by B. ovis and 24 non-infected. We collected samples of whole blood on days 7, 15, 30 and 60 post-infection (PI) by B. ovis. Acetylcholinesterase (AChE) activity in the blood increased on days 15 and 60 PI (P < 0.05). Butyrylcholinesterase (BChE) activity in serum increased on days 7 and 60 PI (P < 0.05). An increase in serum free radical levels occurred on days 7, 15 and 60 PI (P < 0.05), and consequently superoxide dismutase activity increased on day 15 PI (P < 0.05). A reduction in catalase activity occurred when the infection became chronic (60 PI). The increase in AChE and BChE characterized a pro-inflammatory response, since these enzymes regulate levels of acetylcholine (ACh) and butyrylcholine (BuSCh), molecules with anti-inflammatory properties. Therefore, with the increase of cholinesterase activity, there was an extracellular reduction of ACh, an inhibitor of several inflammatory mediators. This proinflammatory response of B. ovis infection leads to oxidative stress, and consequently to cellular damage.
乙酰胆碱酯酶和丁酰胆碱酯酶在绵羊布鲁氏菌感染中的作用
在几种感染性疾病中已经描述了胆碱酯酶在炎症反应中的作用。然而，在布鲁氏菌属中尚未对此进行研究。因此，本研究的目的是评估绵羊布鲁氏菌感染是否改变了对疾病的促炎或抗炎反应中的胆碱能活性。为此，本研究使用了 48 只小鼠，其中 24 只感染了绵羊布鲁氏菌，24 只未感染。我们在感染绵羊布鲁氏菌后的第 7、15、30 和 60 天收集了全血样本。感染后第 15 天和第 60 天，血液中的乙酰胆碱酯酶（AChE）活性增加（P<0.05）。血清中的丁酰胆碱酯酶（BChE）活性在第 7 天和第 60 天增加（P<0.05）。血清自由基水平在第 7、15 和 60 天增加（P<0.05），因此超氧化物歧化酶活性在第 15 天增加（P<0.05）。当感染变为慢性时（第 60 天），过氧化氢酶活性降低。AChE 和 BChE 的增加表明存在促炎反应，因为这些酶调节乙酰胆碱（ACh）和丁酰胆碱（BuSCh）的水平，而这些分子具有抗炎特性。因此，随着胆碱酯酶活性的增加，细胞外的 ACh 减少，ACh 是几种炎症介质的抑制剂。绵羊布鲁氏菌感染的这种促炎反应导致氧化应激，进而导致细胞损伤。
