De Leon-Rodriguez Luis, Lubag Angelo Josue M, Sherry A Dean
Departamento de Quimica. Universidad de Guanajuato. Cerro de la Venada S.N. Col. Pueblito de Rocha., Guanajuato, Gto. Mexico, C.P, 36040.
Inorganica Chim Acta. 2012 Dec 1;393:12-23. doi: 10.1016/j.ica.2012.06.026. Epub 2012 Jun 25.
Our ever-expanding knowledge about the role of zinc in biology includes its role in redox modulation, immune response, neurotransmission, reproduction, diabetes, cancer, and Alzheimers disease is galvanizing interest in detecting and monitoring the various forms of Zn(II) in biological systems. This paper reviews reported strategies for detecting and tracking of labile or "free" unchelated Zn(II) in tissues. While different bound structural forms of Zn(II) have been identified and studied in vitro by multiple techniques, very few molecular imaging methods have successfully tracked the ion in vivo. A number of MRI and optical strategies have now been reported for detection of free Zn(II) in cells and tissues but only a few have been applied successfully in vivo. A recent report of a MRI sensor for in vivo tracking of Zn(II) released from pancreatic β-cells during insulin secretion exemplifies the promise of rational design of new Zn(II) sensors for tracking this biologically important ion in vivo. Such studies promise to provide new insights into zinc trafficking in vivo and the critical role of this ion in many human diseases.
我们对锌在生物学中作用的认识不断扩展，其中包括它在氧化还原调节、免疫反应、神经传递、生殖、糖尿病、癌症以及阿尔茨海默病中的作用，这激发了人们对检测和监测生物系统中各种形式锌离子（Zn(II)）的兴趣。本文综述了已报道的用于检测和追踪组织中不稳定或“游离”未螯合锌离子（Zn(II)）的策略。虽然通过多种技术已在体外鉴定和研究了锌离子（Zn(II)）的不同结合结构形式，但很少有分子成像方法能在体内成功追踪该离子。目前已有一些磁共振成像（MRI）和光学策略用于检测细胞和组织中的游离锌离子（Zn(II)），但只有少数在体内成功应用。最近有一篇关于一种磁共振成像传感器的报道，该传感器用于在体内追踪胰岛素分泌过程中胰腺β细胞释放的锌离子（Zn(II)），这例证了合理设计新型锌离子（Zn(II)）传感器以在体内追踪这种具有重要生物学意义离子的前景。此类研究有望为体内锌离子转运以及该离子在许多人类疾病中的关键作用提供新的见解。
