Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering, Vienna, Austria.
Trends Biotechnol. 2013 Jan;31(1):37-44. doi: 10.1016/j.tibtech.2012.10.008. Epub 2012 Nov 23.
Industrial bioprocesses are commonly based on empiricism rather than scientific process understanding. In this review, we summarize current strategies for science-based bioprocess design and control for filamentous fungi aiming at reducing development times and increasing process economics. We discuss recent developments and trends regarding three crucial aspects throughout the bioprocess life cycle of filamentous fungi, namely (i) strain and inoculum characterization, (ii) morphology, and (iii) rheology, as well as their effects on process performance. Complex interconnections between strain, inoculum, morphology, rheology, and process design are outlined and discussed. Only combining different hard type sensors with soft sensor technology and the development of simplified mechanistic models can enable science-based bioprocess design for filamentous fungi.
工业生物过程通常基于经验主义，而不是对科学过程的理解。在这篇综述中，我们总结了基于科学的丝状真菌生物过程设计和控制的当前策略，旨在缩短开发时间并提高过程经济性。我们讨论了在丝状真菌的整个生物过程生命周期中三个关键方面的最新进展和趋势，即（i）菌株和接种物的特性、（ii）形态和（iii）流变学，以及它们对过程性能的影响。概述并讨论了菌株、接种物、形态、流变学和过程设计之间复杂的相互关系。只有将不同的硬式传感器与软传感器技术结合起来，并开发简化的机理模型，才能实现基于科学的丝状真菌生物过程设计。
