Australian Dental Manufacturing, Brisbane, Qld, Australia.
Int Endod J. 2013 Jun;46(6):530-7. doi: 10.1111/iej.12021. Epub 2012 Nov 27.
To investigate the chemical interaction of calcium hydroxide with the antibiotics demeclocycline calcium in Ledermix Paste and clindamycin hydrochloride in Odontopaste.
Validated methods were developed to analyse the interaction of calcium hydroxide in two forms, Pulpdent and calcium hydroxide powder, with the two antibiotics. High-performance liquid chromatography (HPLC) was used to analyse the mixed samples of the pastes and calcium hydroxide. The concentration of demeclocycline calcium over 0-, 1-, 18-, 24-, 72-h and 7-day time-points was determined. The concentration of clindamycin hydrochloride over 1-, 6-, 24-, 72-h and 7-day time-points was determined. All tests with HPLC involved testing of the standard in duplicate alongside the samples. Linearity, precision and specificity of the testing procedures and apparatus were validated. Descriptive statistics are provided.
The antibiotics in both Odontopaste and Ledermix Paste were affected by the addition of calcium hydroxide. When mixed with calcium hydroxide powder, Odontopaste had a 2% loss of clindamycin hydrochloride over 7 days, but when mixed with Pulpdent, there was a 36% loss over 7 days. Ledermix Paste showed an 80% loss of demeclocycline calcium over 7 days when mixed with calcium hydroxide powder and a 19% loss when mixed with Pulpdent over the 7-day period.
The addition of calcium hydroxide to Odontopaste or Ledermix Paste results in reductions of the respective antibiotic over a 7-day time period.
研究氢氧化钙与雷得美士糊剂中的地美环素钙和奥得典糊剂中的盐酸克林霉素的化学相互作用。
建立了两种形式的氢氧化钙（派丽登和氢氧化钙粉）与两种抗生素相互作用的验证方法。采用高效液相色谱法（HPLC）分析糊剂与氢氧化钙混合样品。测定了地美环素钙在 0、1、18、24、72 小时和 7 天时间点的浓度。测定了盐酸克林霉素在 1、6、24、72 小时和 7 天时间点的浓度。所有 HPLC 测试均包括标准品和样品的重复测试。验证了测试程序和仪器的线性、精密度和特异性。提供了描述性统计数据。
奥得典糊剂和雷得美士糊剂中的抗生素均受到添加氢氧化钙的影响。当与氢氧化钙粉混合时，奥得典糊剂中盐酸克林霉素在 7 天内损失 2%，但与派丽登混合时，7 天内损失 36%。当与氢氧化钙粉混合时，雷得美士糊剂中地美环素钙在 7 天内损失 80%，而与派丽登混合时则在 7 天内损失 19%。
奥得典糊剂或雷得美士糊剂中添加氢氧化钙会导致相应抗生素在 7 天内减少。
