Duo Rui, Chen Yan, Liu Yuhong, Huang Zhifang, Liu Yunhua, Yi Jinhai
Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
Zhongguo Zhong Yao Za Zhi. 2012 Aug;37(15):2313-6.
To determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.
By HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.
Carboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).
This method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.
采用高效液相色谱法测定苍耳子中羧基苍术苷和苍术苷的含量。
采用高效液相色谱法，色谱柱为Agilent ZORBAX SB -苯基柱（4.6 mm×250 mm，5 µm），以乙腈 - 0.01 mol·L⁻¹磷酸二氢钠（pH 6）为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹。检测波长为203 nm，柱温设定为35℃。
羧基苍术苷在0.0972 - 1.944 μg范围内线性关系良好，苍术苷在0.1030 - 2.060 μg范围内线性关系良好。羧基苍术苷的回收率为100.3%，苍术苷的回收率为102.5%。相对标准偏差分别为0.67%和1.4%（n = 6）。
该方法简便、实用、重复性好，可用于苍耳子的质量控制。
