Liu Min, Zhou Xiao-jiang
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1783-6.
An HPLC method was established to simultaneously determine the five sesquiterpene lactones, carabrone, carabrol, 2-desoxy-4-epi-pulchellin, telekinand 11(13)-dehydroivaxillin in Carpesium abrotanoides. Samples were analyzed on a kromasil C18 column (4.6 mm x 250 mm, 5 µm); mobile phase: A was acetonitrile, B was water, with gradient elution; flow speed: 1.0 mL · min(-1); detection was carried out using a photodiode array detector at 211 nm; temperatureof column: 30 °C. The five sesquiterpene lactones were well separated with good linear correlations in the range of 0.270-2.700 (r = 0.999 7 ), 1.336-13.360 (r = 0.999 6), 0.258-2.580 (r = 0.999 7), 0.238-2.380 (r = 0.999 9), 0.490-4.900 µg (r = 0.999 9) for carabrone, carabrol, 2-desoxy-4-epi-pulchellin, telekin and 11 (13) -dehydroivaxillin. The average recoveries of these five sesquiterpene lactoneswere 96.78%-98.41% (RSD 1.3%-2.9%). The method was simple, repeatable and stable, which could be used for quality control of C. abrotanoide.
建立了一种高效液相色谱法同时测定天名精中5种倍半萜内酯，即卡拉酮、卡拉酚、2-脱氧-4-表-普尔切林、特勒金和11(13)-脱氢艾瓦西林。样品在kromasil C18柱（4.6 mm×250 mm，5 µm）上进行分析；流动相：A为乙腈，B为水，采用梯度洗脱；流速：1.0 mL·min(-1)；使用光电二极管阵列检测器在211 nm波长处进行检测；柱温：30℃。这5种倍半萜内酯得到了很好的分离，在0.270 - 2.700（r = 0.999 7）、1.336 - 13.360（r = 0.999 6）、0.258 - 2.580（r = 0.999 7）、0.238 - 2.380（r = 0.999 9）、0.490 - 4.900 µg（r = 0.999 9）范围内线性关系良好，分别对应卡拉酮、卡拉酚、2-脱氧-4-表-普尔切林、特勒金和11(13)-脱氢艾瓦西林。这5种倍半萜内酯的平均回收率为96.78% - 98.41%（相对标准偏差1.3% - 2.9%）。该方法简便、可重复且稳定，可用于天名精的质量控制。
