Baraniuk J N, Lundgren J D, Goff J, Peden D, Merida M, Shelhamer J, Kaliner M
Allergic Diseases Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
J Clin Invest. 1990 Apr;85(4):998-1005. doi: 10.1172/JCI114577.
Gastrin-releasing peptide (GRP), the 27 amino acid mammalian form of bombesin, was studied in human inferior turbinate nasal mucosa. The GRP content of the mucosa measured by radioimmunoassay was 0.60 +/- 0.25 pmol/g tissue (n = 9 patients; mean +/- SEM). GRP-immunoreactive nerves detected by the immunogold method of indirect immunohistochemistry were found predominantly in small muscular arteries, arterioles, venous sinusoids, and between submucosal gland acini. 125I-GRP binding sites determined by autoradiography were exclusively and specifically localized to nasal epithelium and submucosal glands. There was no binding to vessels. The effects of GRP on submucosal gland product release were studied in short-term explant culture. GRP (10 microM) significantly stimulated the release of the serous cell-specific product lactoferrin, and [3H]glucosamine-labeled glycoconjugates which are products of epithelial goblet cells and submucosal gland cells. These observations indicate that GRP released from nerve fibers probably acts on glandular GRP receptors to induce glycoconjugate release from submucosal glands and epithelium and lactoferrin release from serous cells, but that GRP would probably not affect vascular permeability.
胃泌素释放肽（GRP），即27个氨基酸的哺乳动物形式的蛙皮素，在人下鼻甲鼻黏膜中进行了研究。通过放射免疫测定法测得的黏膜GRP含量为0.60±0.25 pmol/g组织（n = 9例患者；均值±标准误）。通过间接免疫组织化学的免疫金法检测到的GRP免疫反应性神经主要存在于小肌动脉、小动脉、静脉窦以及黏膜下腺泡之间。通过放射自显影法确定的125I-GRP结合位点仅特异性地定位于鼻上皮和黏膜下腺。血管无结合。在短期外植体培养中研究了GRP对黏膜下腺产物释放的影响。GRP（10 μM）显著刺激了浆液细胞特异性产物乳铁蛋白以及[3H]葡萄糖胺标记的糖缀合物的释放，后者是上皮杯状细胞和黏膜下腺细胞的产物。这些观察结果表明，从神经纤维释放的GRP可能作用于腺GRP受体，以诱导黏膜下腺和上皮释放糖缀合物以及浆液细胞释放乳铁蛋白，但GRP可能不会影响血管通透性。
