Tao Pingping, Zheng Weiping, Wang Yungen, Bian Mei-Lu
Department of Gynecology, Shaoxing People's Hospital and Shaoxing Hospital of Zhejiang University, Shaoxing 312000, China.
J Biomed Biotechnol. 2012;2012:938780. doi: 10.1155/2012/938780. Epub 2012 Oct 11.
Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH). 591 women were classified into 6 groups according to their histological diagnoses. The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group. The overall accordance was excellent with the Kappa index (KI) of 0.814. The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75 > KI > 0.4, good; KI ≥ 0.75, excellent). The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls. In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use.
高危型人乳头瘤病毒（HPV）的持续感染已被确认为宫颈癌的直接病因。因此，HPV检测及基因分型对宫颈癌筛查至关重要。在本研究中，我们通过将流通杂交基因芯片技术（HybriMax）的结果与第二代杂交捕获法（HC-II）及原位杂交（ISH）的结果相比较，评估了该技术在HPV基因分型方面的效能。591名女性根据组织学诊断被分为6组。在癌症组中，HybriMax与HC-II对13种HPV基因型的总体符合率分别为92.5%和100%。总体符合情况极佳，Kappa指数（KI）为0.814。每组的KI值分别为0.750（正常细胞学诊断）、0.781（慢性宫颈炎）、0.80（尖锐湿疣）、0.755（宫颈上皮内瘤变（CIN）I级）、0.723（CIN II级）和0.547（CIN III级）（0.75＞KI＞0.4，良好；KI≥0.75，极佳）。HybriMax检测出的患者中最常见的10种HPV亚型为16、52/58、18、33、31、81、53、68和66，正常对照中为16、68、18、52、58、11、53、31/39和33。总之，HybriMax是一种有效的HPV基因分型方法，更适合临床应用。
