Department of Animal Pathology and Health, University of Naples Federico II, Naples, Italy.
Parasite. 2012 Nov;19(4):437-40. doi: 10.1051/parasite/2012194437.
In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryptosporidium oocysts in nine of the 150 samples examined (6.0%), precisely in 6/125 snakes (4.8%) and in 3/25 lizards (12.0%); all fecal samples tested negative for the presence of Giardia cysts. Molecular characterization based on nested PCR amplification and sequencing of the SSU-rRNA gene, revealed the presence of Cryptosporidium serpentis in three samples from snakes (Boa constrictor constrictor, Elapheguttata guttata guttata and Python molurus).
为了获取在意大利南部作为宠物饲养的圈养蜥蜴和蛇类中隐孢子虫感染的流行情况和遗传数据，对 150 只爬行动物进行了调查。粪便样本保存在 5%的福尔马林中，并使用商业免疫荧光检测法（IFA）检测隐孢子虫卵囊和贾第虫囊肿。IFA 在检查的 150 个样本中的 9 个（6.0%）中发现了隐孢子虫卵囊，准确地说，在 125 条蛇中的 6 条（4.8%）和 25 条蜥蜴中的 3 条（12.0%）中发现了隐孢子虫卵囊；所有粪便样本均未检测到贾第虫囊肿的存在。基于巢式 PCR 扩增和 SSU-rRNA 基因测序的分子特征表明，在来自蛇的三个样本中存在蛇隐孢子虫（Boa constrictor constrictor、Elaphe guttata guttata guttata 和 Python molurus）。
