意大利宠物蜥蜴和蛇体内隐孢子虫分离株的流行情况和分子鉴定。
Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy.
机构信息
Department of Animal Pathology and Health, University of Naples Federico II, Naples, Italy.
出版信息
Parasite. 2012 Nov;19(4):437-40. doi: 10.1051/parasite/2012194437.
Abstract
In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryptosporidium oocysts in nine of the 150 samples examined (6.0%), precisely in 6/125 snakes (4.8%) and in 3/25 lizards (12.0%); all fecal samples tested negative for the presence of Giardia cysts. Molecular characterization based on nested PCR amplification and sequencing of the SSU-rRNA gene, revealed the presence of Cryptosporidium serpentis in three samples from snakes (Boa constrictor constrictor, Elapheguttata guttata guttata and Python molurus).
摘要
为了获取在意大利南部作为宠物饲养的圈养蜥蜴和蛇类中隐孢子虫感染的流行情况和遗传数据，对 150 只爬行动物进行了调查。粪便样本保存在 5%的福尔马林中，并使用商业免疫荧光检测法（IFA）检测隐孢子虫卵囊和贾第虫囊肿。IFA 在检查的 150 个样本中的 9 个（6.0%）中发现了隐孢子虫卵囊，准确地说，在 125 条蛇中的 6 条（4.8%）和 25 条蜥蜴中的 3 条（12.0%）中发现了隐孢子虫卵囊；所有粪便样本均未检测到贾第虫囊肿的存在。基于巢式 PCR 扩增和 SSU-rRNA 基因测序的分子特征表明，在来自蛇的三个样本中存在蛇隐孢子虫（Boa constrictor constrictor、Elaphe guttata guttata guttata 和 Python molurus）。
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1
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BMC Vet Res. 2012 May 28;8:69. doi: 10.1186/1746-6148-8-69.
2
FLOTAC can detect parasitic and pseudoparasitic elements in reptiles.FLOTAC 可用于检测爬行动物中的寄生和拟寄生生物。
Exp Parasitol. 2012 Mar;130(3):282-4. doi: 10.1016/j.exppara.2012.01.011. Epub 2012 Jan 27.
3
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J Vet Diagn Invest. 2011 May;23(3):430-5. doi: 10.1177/1040638711403415.
4
Zoonotic enteric protozoa.动物源肠道原生动物。
Vet Parasitol. 2011 Nov 24;182(1):70-8. doi: 10.1016/j.vetpar.2011.07.016. Epub 2011 Jul 12.
5
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Vet Rec. 2011 Aug 20;169(8):207. doi: 10.1136/vr.d4398. Epub 2011 Jul 27.
6
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Acta Vet Scand. 2011 May 30;53(1):33. doi: 10.1186/1751-0147-53-33.
7
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Vet Parasitol. 2011 Jan 10;175(1-2):165-7. doi: 10.1016/j.vetpar.2010.10.002. Epub 2010 Oct 30.
8
Evidence for a new species of Cryptosporidium infecting tortoises: Cryptosporidium ducismarci.感染陆龟的一种新型隐孢子虫——杜氏隐孢子虫的证据。
Parasit Vectors. 2010 Mar 25;3:21. doi: 10.1186/1756-3305-3-21.
9
FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans.FLOTAC：用于定性和定量检测动物和人类粪便中寄生虫的新型多价技术。
Nat Protoc. 2010 Mar;5(3):503-15. doi: 10.1038/nprot.2009.235. Epub 2010 Feb 25.
10
Molecular characterisation of Cryptosporidium isolates from pet reptiles.来自宠物爬行动物的隐孢子虫分离株的分子特征分析。
Vet Parasitol. 2009 Mar 23;160(3-4):204-10. doi: 10.1016/j.vetpar.2008.11.003. Epub 2008 Nov 13.
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