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FLOTAC:用于定性和定量检测动物和人类粪便中寄生虫的新型多价技术。

FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans.

机构信息

Department of Pathology and Animal Health, Faculty of Veterinary Medicine, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy.

出版信息

Nat Protoc. 2010 Mar;5(3):503-15. doi: 10.1038/nprot.2009.235. Epub 2010 Feb 25.

Abstract

Accurate diagnosis of parasitic infections is of pivotal importance for both individual patient management and population-based studies, such as drug efficacy trials and surveillance of parasitic disease control and elimination programs, in both human and veterinary public health. In this study, we present protocols for the FLOTAC basic, dual and double techniques, which are promising new multivalent, sensitive, accurate and precise methods for qualitative and quantitative copromicroscopic analysis. These various methods make use of the FLOTAC apparatus, a cylindrical device with two 5-ml flotation chambers, which allows up to 1 g of stool to be prepared for microscopic analysis. Compared with currently more widely used diagnostic methods for parasite detection in animals (e.g., McMaster and Wisconsin techniques) and humans (e.g., Kato-Katz and ether-based concentration techniques), the FLOTAC techniques show higher sensitivity and accuracy. All FLOTAC techniques can be performed on fresh fecal material as well as preserved stool samples, and require approximately 12-15 min of preparation time before microscopic analysis.

摘要

寄生虫感染的准确诊断对于个体患者管理和基于人群的研究都至关重要,这些研究包括药物疗效试验以及寄生虫病控制和消除规划的监测,无论是在人类还是兽医公共卫生领域。在本研究中,我们介绍了 FLOTAC 基本法、双槽法和双槽双染法的方案,这些都是很有前途的新的多价、敏感、准确和精确的定性和定量粪便显微镜分析方法。这些不同的方法都利用了 FLOTAC 仪器,这是一种带有两个 5 毫升浮选室的圆柱形装置,可用于制备 1 克粪便进行显微镜分析。与目前在动物(例如,麦克马斯特和威斯康星技术)和人类(例如,加藤和乙醚浓缩技术)中更广泛使用的寄生虫检测诊断方法相比,FLOTAC 技术显示出更高的灵敏度和准确性。所有的 FLOTAC 技术都可以应用于新鲜粪便样本和保存的粪便样本,并且在进行显微镜分析之前,大约需要 12-15 分钟的准备时间。

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