School of Chemical Engineering, Sichuan University, 610065 Chengdu, China.
Bioresour Technol. 2013 Jan;128:246-51. doi: 10.1016/j.biortech.2012.10.077. Epub 2012 Oct 26.
Acetone-butanol-ethanol (ABE) fermentation by combining a PDMS membrane bioreactor and Clostridium acetobutylicum was studied, and a long continuous and closed-circulating fermentation (CCCF) system has been achieved. Two cycles of experiment were conducted, lasting for 274 h and 300 h, respectively. The operation mode of the first cycle was of fermentation intermittent coupling with pervaporation, and the second cycle was of continuous coupling. The average cell weight, glucose consumption rate, butanol productivity and butanol production of the first cycle were 1.59 g L(-1), 0.63 g L(-1)h(-1), 0.105 g L(-1)h(-1) and 28.03 g L(-1), respectively. Correspondingly, the four parameters of the second cycle were 1.68 g L(-1), 1.12 g L(-1)h(-1), 0.205 g L(-1)h(-1) and 61.43 g L(-1), respectively. The results indicate the fermentation behaviors under continuous coupling mode were superior to that under intermittent coupling mode. Besides, two peak values were observed in the time course profiles, which means the microorganism could adapt the long CCCF membrane bioreactor system.
采用聚二甲基硅氧烷（PDMS）膜生物反应器结合丙酮丁醇乙醇（ABE）发酵梭菌，实现了长连续封闭循环发酵（CCCF）系统。进行了两轮实验，分别持续 274 小时和 300 小时。第一周期的操作模式为发酵与渗透蒸发间歇耦合，第二周期为连续耦合。第一周期的平均细胞重量、葡萄糖消耗率、丁醇生产率和丁醇产量分别为 1.59 g/L、0.63 g/L/h、0.105 g/L/h 和 28.03 g/L。相应地，第二周期的四个参数分别为 1.68 g/L、1.12 g/L/h、0.205 g/L/h 和 61.43 g/L。结果表明，连续耦合模式下的发酵行为优于间歇耦合模式。此外，在时间过程曲线中观察到两个峰值，这意味着微生物可以适应长 CCCF 膜生物反应器系统。
