Chen Chunyan, Long Sihua, Li Airong, Xiao Guoqing, Wang Linyuan, Xiao Zeyi
a College of Chemistry and Chemical Engineering, Southwest Petroleum University , Chengdu , China.
b State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Southwest Petroleum University , Chengdu , China.
Prep Biochem Biotechnol. 2017 Mar 16;47(3):254-260. doi: 10.1080/10826068.2016.1224242. Epub 2016 Aug 24.
Since both ethanol and butanol fermentations are urgently developed processes with the biofuel-demand increasing, performance comparison of aerobic ethanol fermentation and anerobic butanol fermentation in a continuous and closed-circulating fermentation (CCCF) system was necessary to achieve their fermentation characteristics and further optimize the fermentation process. Fermentation and pervaporation parameters including the average cell concentration, glucose consumption rate, cumulated production concentration, product flux, and separation factor of ethanol fermentation were 11.45 g/L, 3.70 g/L/h, 655.83 g/L, 378.5 g/m/h, and 4.83, respectively, the corresponding parameters of butanol fermentation were 2.19 g/L, 0.61 g/L/h, 28.03 g/L, 58.56 g/m/h, and 10.62, respectively. Profiles of fermentation and pervaporation parameters indicated that the intensity and efficiency of ethanol fermentation was higher than butanol fermentation, but the stability of butanol fermentation was superior to ethanol fermentation. Although the two fermentation processes had different features, the performance indicated the application prospect of both ethanol and butanol production by the CCCF system.
由于随着生物燃料需求的增加，乙醇发酵和丁醇发酵都是亟待发展的工艺，因此有必要在连续闭路循环发酵（CCCF）系统中对好氧乙醇发酵和厌氧丁醇发酵的性能进行比较，以了解它们的发酵特性，并进一步优化发酵过程。乙醇发酵的发酵和渗透蒸发参数，包括平均细胞浓度、葡萄糖消耗速率、累积产物浓度、产物通量和分离因子，分别为11.45 g/L、3.70 g/L/h、655.83 g/L、378.5 g/m/h和4.83，丁醇发酵的相应参数分别为2.19 g/L、0.61 g/L/h、28.03 g/L、58.56 g/m/h和10.62。发酵和渗透蒸发参数曲线表明，乙醇发酵的强度和效率高于丁醇发酵，但丁醇发酵的稳定性优于乙醇发酵。尽管这两种发酵工艺具有不同的特点，但该性能表明了CCCF系统生产乙醇和丁醇的应用前景。
