College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, China; College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, China.
Bioresour Technol. 2014 Jul;163:6-11. doi: 10.1016/j.biortech.2014.04.004. Epub 2014 Apr 13.
Two rounds of acetone-butanol-ethanol (ABE) fermentation under slight pressure were carried out in the continuous and closed-circulating fermentation (CCCF) system. Spores of the clostridium were observed and counted, with the maximum number of 2.1 × 10(8) and 2.3 × 10(8)ml(-1) separately. The fermentation profiles were comparable with that at atmospheric pressure, showing an average butanol productivity of 0.14 and 0.13 g L(-1)h(-1). Moreover, the average gas productivities of 0.28 and 0.27 L L(-1)h(-1) were obtained in two rounds of CCCF, and the cumulative gas production of 52.64 and 25.92 L L(-1) were achieved, with the hydrogen volume fraction of 41.43% and 38.08% respectively. The results suggested that slight pressures have no obvious effect on fermentation performance, and also indicated the significance and feasibility of gas recovery in the continuous ABE fermentation process.
在连续闭路发酵(CCCF)系统中进行了两轮轻微压力下的丙酮丁醇乙醇(ABE)发酵。观察并计数了梭菌孢子,分别达到了 2.1×10(8)和 2.3×10(8)ml(-1)的最大值。发酵曲线与常压下的发酵曲线相当,平均丁醇产率分别为 0.14 和 0.13 g L(-1)h(-1)。此外,两轮 CCCF 的平均气体产率分别为 0.28 和 0.27 L L(-1)h(-1),累积气体产量分别为 52.64 和 25.92 L L(-1),氢气体积分数分别为 41.43%和 38.08%。结果表明,轻微的压力对发酵性能没有明显影响,也表明了在连续 ABE 发酵过程中回收气体的重要性和可行性。