Umeki Saori, Ema Yasuo, Suzuki Ryoichi, Kubo Masahito, Hayashi Toshiharu, Okamura Yasuhiko, Yamazaki Jumpei, Tsujimoto Hajime, Tani Kenji, Hiraoka Hiroko, Okuda Masaru, Mizuno Takuya
Laboratory of Veterinary Internal Medicine, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, Japan.
J Vet Med Sci. 2013 May 2;75(4):467-74. doi: 10.1292/jvms.12-0448. Epub 2012 Nov 28.
Five novel, canine lymphoma cell lines (Ema, CLC, CLK, Nody-1 and UL-1) were established from dogs suffering from lymphoma and characterized in vitro and in vivo. All cell lines, except CLC, were characterized with T-cell phenotypes, by flow cytometric analysis and polymerase chain reaction for antigen receptor rearrangement. Cell proliferation rates and transcriptional levels of MYC, PTEN, KIT and FLT3 varied between each cell line. Intraperitoneal xenotransplantation of Ema, CLC, Nody-1 and UL-1 lymphoma cell lines into NOD/SCID mice induced ascites, intraperitoneal tumors and severe infiltration of lymphoma cells into the pancreas and mesentery. Establishment of novel canine lymphoma cell lines with different characteristics is critical for elucidating the pathophysiology of canine lymphoma and improving current therapies.
从患有淋巴瘤的犬只中建立了五个新的犬淋巴瘤细胞系（Ema、CLC、CLK、Nody-1和UL-1），并在体外和体内进行了表征。除CLC外，所有细胞系通过流式细胞术分析和抗原受体重排的聚合酶链反应进行T细胞表型鉴定。各细胞系之间的细胞增殖率以及MYC、PTEN、KIT和FLT3的转录水平各不相同。将Ema、CLC、Nody-1和UL-1淋巴瘤细胞系腹腔内异种移植到NOD/SCID小鼠中可诱导腹水、腹腔肿瘤以及淋巴瘤细胞严重浸润胰腺和肠系膜。建立具有不同特征的新型犬淋巴瘤细胞系对于阐明犬淋巴瘤的病理生理学和改进当前治疗方法至关重要。
