Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Antonie Van Leeuwenhoek. 2013 Apr;103(4):771-6. doi: 10.1007/s10482-012-9859-z. Epub 2012 Nov 30.
A novel halophilic actinomycete, strain H32(T), was isolated from a Saharan soil sample collected in El-Oued province, south Algeria. The isolate was characterized by means of polyphasic taxonomy. Optimal growth was determined to occur at 28-32 °C, pH 6.0-7.0 and in the presence of 15-25 % (w/v) NaCl. The strain was observed to produce abundant aerial mycelium, which formed long chains of rod-shaped spores at maturity, and fragmented substrate mycelium. The cell wall was determined to contain meso-diaminopimelic acid and the characteristic whole-cell sugars were arabinose and galactose. The predominant menaquinones were found to be MK-10(H4) and MK-9(H4). The predominant cellular fatty acids were determined to be anteiso C17:0, iso-C15:0 and iso-C16:0. The diagnostic phospholipid detected was phosphatidylcholine. Phylogenetic analyses based on the 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Actinopolyspora. The 16S rRNA gene sequence similarity indicated that strain H32(T) was most closely related to 'Actinopolyspora algeriensis' DSM 45476(T) (98.8 %) and Actinopolyspora halophila DSM 43834(T) (98.5 %). Furthermore, the result of DNA-DNA hybridization between strain H32(T) and the type strains 'A. algeriensis' DSM 45476(T), A. halophila DSM 43834(T) and Actinopolyspora mortivallis DSM 44261(T) demonstrated that this isolate represents a different genomic species in the genus Actinopolyspora. Moreover, the physiological and biochemical data allowed the differentiation of strain H32(T) from its closest phylogenetic neighbours. Therefore, it is proposed that strain H32(T) represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora saharensis sp. nov. is proposed. The type strain is H32(T) (=DSM 45459(T)=CCUG 62966(T)).
一株嗜盐放线菌 H32(T)，从阿尔及利亚南部 El-Oued 省的撒哈拉土壤样本中分离得到。该分离株通过多相分类学方法进行了特征描述。最适生长条件为 28-32°C、pH6.0-7.0 和 15-25%（w/v）NaCl。该菌株产生丰富的气生菌丝，成熟时形成长链的杆状孢子，并且基质菌丝断裂。细胞壁含有 meso-二氨基庚二酸，特征性的全细胞糖为阿拉伯糖和半乳糖。主要的menaquinones 为 MK-10(H4) 和 MK-9(H4)。主要的细胞脂肪酸为 anteiso C17:0、iso-C15:0 和 iso-C16:0。检测到的诊断性磷脂为磷脂酰胆碱。基于 16S rRNA 基因序列的系统发育分析表明，该菌株在放线菌属的辐射区内形成了一个独特的谱系。16S rRNA 基因序列相似性表明，菌株 H32(T)与 'Actinopolyspora algeriensis' DSM 45476(T)（98.8%）和 Actinopolyspora halophila DSM 43834(T)（98.5%）最为密切相关。此外，菌株 H32(T)与模式菌株 'A. algeriensis' DSM 45476(T)、A. halophila DSM 43834(T) 和 Actinopolyspora mortivallis DSM 44261(T) 之间的 DNA-DNA 杂交结果表明，该分离株代表了放线菌属中的一个不同的基因组种。此外，生理生化数据使菌株 H32(T)与最接近的系统发育近邻区分开来。因此，建议菌株 H32(T)代表放线菌属的一个新种，命名为 Actinopolyspora saharensis sp. nov.。模式菌株为 H32(T)（=DSM 45459(T)=CCUG 62966(T)）。
