Cereal Research Centre, Agriculture and AgriFood Canada, 195 Dafoe Road, Winnipeg, Manitoba, Canada.
Proteomics. 2013 Feb;13(3-4):579-96. doi: 10.1002/pmic.201200270. Epub 2013 Jan 14.
It has been clearly demonstrated that plants redox control can be exerted over virtually every cellular metabolic pathway affecting metabolic homeostasis and energy balance. Therefore, a tight link exists between cellular/compartmental steady-state redox level and cellular metabolism. Proteomics offers a powerful new way to characterize the response and regulation of protein oxidation in different cell types and in relation to cellular metabolism. Compelling evidence revealed in proteomics studies suggests the integration of the redox network with other cellular signaling pathways such as Ca(2+) and/or protein phosphorylation, jasmonic, salicylic, abscisic acids, ethylene, and other phytohormones. Here we review progress in using the various proteomics techniques and approaches to answer biological questions arising from redox signaling and from changes in redox status of the cell. The focus is on reversible redox protein modifications and on three main processes, namely oxidative and nitrosative stress, defense against pathogens, cellular redox response and regulation, drawing on examples from plant redox proteomics studies.
已经清楚地表明，植物的氧化还原控制可以作用于几乎每一种影响代谢稳态和能量平衡的细胞代谢途径。因此，细胞/区室稳态氧化还原水平与细胞代谢之间存在紧密联系。蛋白质组学为研究不同细胞类型中蛋白质氧化的反应和调节提供了一种强大的新方法，以及与细胞代谢的关系。蛋白质组学研究中揭示的令人信服的证据表明，氧化还原网络与其他细胞信号通路（如 Ca(2+)和/或蛋白磷酸化、茉莉酸、水杨酸、脱落酸、乙烯和其他植物激素）的整合。在这里，我们回顾了使用各种蛋白质组学技术和方法来回答由氧化还原信号和细胞氧化还原状态变化引起的生物学问题的进展。重点是可逆的氧化还原蛋白修饰以及三个主要过程，即氧化和硝化应激、抵御病原体、细胞氧化还原反应和调节，这三个过程主要是从植物氧化还原蛋白质组学研究中举例说明的。
