Pegu Seema R, Das Pranab Jyoti, Sonowal Joyshikh, Sengar Gyanendra Singh, Deb Rajib, Yadav Ajay Kumar, Rajkhowa Swaraj, Choudhury Manjisa, Gulati Baldev R, Gupta Vivek Kumar
ICAR-National Research Centre on Pig, Rani, Guwahati 781131, India.
ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
Vaccines (Basel). 2023 Jan 10;11(1):150. doi: 10.3390/vaccines11010150.
Japanese encephalitis viruses (JEVs) are globally prevalent as deadly pathogens in humans and animals, including pig, horse and cattle. Japanese encephalitis (JE) still remains an important cause of epidemic encephalitis worldwide and exists in a zoonotic transmission cycle. Assam is one of the highly endemic states for JE in India. In the present study, to understand the epidemiological status of JE circulating in pigs and mosquito, particularly in Assam, India, molecular detection of JEV and the genome sequencing of JEV isolates from pigs and mosquitoes was conducted. The genome analysis of two JEV isolates from pigs and mosquitoes revealed 7 and 20 numbers of unique points of polymorphism of nucleotide during alignment of the sequences with other available sequences, respectively. Phylogenetic analysis revealed that the isolates of the present investigation belong to genotype III and are closely related with the strains of neighboring country China. This study highlights the transboundary nature of the JEV genotype III circulation, which maintained the same genotype through mosquito-swine transmission cycles.
日本脑炎病毒（JEVs）作为人类和动物（包括猪、马和牛）的致命病原体在全球广泛流行。日本脑炎（JE）仍是全球流行性脑炎的一个重要病因，存在于人畜共患病传播循环中。阿萨姆邦是印度JE的高流行地区之一。在本研究中，为了解在猪和蚊子中传播的JE的流行病学状况，特别是在印度阿萨姆邦，对JEV进行了分子检测，并对从猪和蚊子中分离出的JEV进行了基因组测序。对从猪和蚊子中分离出的两个JEV毒株进行的基因组分析显示，在将序列与其他可用序列比对时，核苷酸多态性的独特位点分别有7个和20个。系统发育分析表明，本研究中的分离株属于基因型III，与邻国中国的毒株密切相关。本研究突出了JEV基因型III传播的跨界性质，其通过蚊子-猪传播循环保持相同的基因型。
