Department of Molecular Biotechnology, School of Life & Environmental Sciences, Konkuk University, Seoul, Republic of Korea.
Plant Sci. 2013 Jan;198:7-16. doi: 10.1016/j.plantsci.2012.09.014. Epub 2012 Oct 5.
Manganese stabilizing protein (MSP) is an important component of the Photosystem II (PSII) oxygen evolving complex. In our previous work, transgenic potato plants with reduced expression of MSP (MSP-As) were developed and their physiological and biochemical responses were studied. In this report, we address the response of MSP-As plants toward salinity, heavy metal and osmotic stresses. MSP-As plants treated with NaCl, ZnCl(2) or mannitol solution showed significant level of tolerance under all the stress conditions. Specific enzyme activities of major ROS-scavenging enzymes were found significantly higher in MSP-As plants than the control plants. MSP-As plants accumulated increased levels of proline and low molecular weight metabolites such as ascorbate and α-tocopherol, which indicated that these plants were much more resistant to stress compared to the corresponding control plants. The primary photochemical efficiencies and the OJIP kinetics analyses further confirmed that MSP-As plants were in better optimal health under stress compared to the control plants. Although the exact reason behind the increased stress tolerance in stressed MSP-As plants is unclear, our results strongly indicate the role of MSP of unknown function in abiotic stress tolerance.
锰稳定蛋白（MSP）是光系统 II（PSII）放氧复合物的重要组成部分。在我们之前的工作中，已经开发出表达减少的 MSP 的转基因马铃薯植株，并研究了它们的生理和生化反应。在本报告中，我们研究了 MSP-As 植物对盐度、重金属和渗透胁迫的反应。用 NaCl、ZnCl2 或甘露醇溶液处理的 MSP-As 植物在所有胁迫条件下均表现出显著的耐受性。在 MSP-As 植物中发现主要 ROS 清除酶的特定酶活性明显高于对照植物。MSP-As 植物积累了更多的脯氨酸和低分子量代谢物，如抗坏血酸和α-生育酚，这表明与相应的对照植物相比，这些植物对胁迫有更强的抗性。初级光化学效率和 OJIP 动力学分析进一步证实，与对照植物相比，MSP-As 植物在胁迫下处于更好的最佳健康状态。虽然在胁迫的 MSP-As 植物中增加的胁迫耐受性的确切原因尚不清楚，但我们的结果强烈表明，未知功能的 MSP 在非生物胁迫耐受性中发挥作用。
