Integrative Oncology Department, BC Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3.
Photodiagnosis Photodyn Ther. 2012 Dec;9(4):299-302. doi: 10.1016/j.pdpdt.2012.02.001. Epub 2012 May 31.
Here we present a method for improving Raman spectroscopy signal-to-noise ratio (SNR) based on fluorescence photobleaching. Good SNR is essential to obtain biochemical information about biological tissues. Subtracting high levels of tissue autofluorescence background is a major challenge in extracting weak Raman signals. We found that pre-exposure to laser light significantly reduces tissue autofluorescence, but minimally affects Raman signals, allowing subsequent acquisition of high-SNR Raman spectra. We demonstrated this method with in vivo Raman spectral measurements of human skin. This method will benefit clinical skin Raman measurements of body sites with high autofluorescence background such as the forehead and nose.
在这里，我们提出了一种基于荧光漂白的提高拉曼光谱信噪比（SNR）的方法。良好的 SNR 对于获取生物组织的生化信息至关重要。从提取弱拉曼信号中减去组织自发荧光的高强度背景是一个主要挑战。我们发现，预先暴露于激光光会显著降低组织自发荧光，但对拉曼信号的影响最小，从而允许随后获得高 SNR 的拉曼光谱。我们通过对人体皮肤的体内拉曼光谱测量验证了这种方法。这种方法将有益于前额和鼻子等具有高自发荧光背景的身体部位的临床皮肤拉曼测量。
