Sarin V K, Gupta S, Leung T K, Taylor V E, Ohning B L, Whitsett J A, Fox J L
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Proc Natl Acad Sci U S A. 1990 Apr;87(7):2633-7. doi: 10.1073/pnas.87.7.2633.
We have synthesized pulmonary surfactant apoprotein SP-B peptides by solid-phase chemistry and demonstrated their ability to enhance the surface-active properties of synthetic lipid mixtures. The synthetic peptides were reactive with antiserum generated against the native bovine surfactant peptide. Both peptides conferred surfactant-like properties to synthetic lipid mixtures as assessed by a Wilhelmy balance and pulsating bubble surfactometer. Likewise, mixtures of synthetic SP-B peptides and lipid restored compliance of isolated surfactant-deficient rat lungs. This work demonstrates the utility of SP-B as a functional component of pulmonary surfactant mixtures for treatment of respiratory distress syndrome or other disorders characterized by surfactant deficiency.
我们通过固相化学合成了肺表面活性物质载脂蛋白SP - B肽，并证明了它们增强合成脂质混合物表面活性的能力。合成肽与针对天然牛表面活性肽产生的抗血清发生反应。通过威尔海姆天平（Wilhelmy balance）和脉动气泡表面张力仪评估，这两种肽都赋予合成脂质混合物类似表面活性剂的特性。同样，合成SP - B肽与脂质的混合物恢复了分离出的表面活性剂缺乏的大鼠肺的顺应性。这项工作证明了SP - B作为肺表面活性物质混合物的功能成分在治疗呼吸窘迫综合征或其他以表面活性剂缺乏为特征的疾病中的实用性。
