Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
Eur Rev Med Pharmacol Sci. 2012 Nov;16(13):1840-6.
Follicular fluid (FF) is biological fluid rich in nutrients, growth factors, hormones and may affect the sperm quality. Sperm washing has been done using conventional media in laboratory procedure so far.
This study aimed to investigate the effects of FF on survival and maintenance of chromatin integrity post swim up.
Each washed semen sample was divided into two parts; the control group was incubated in the media, and the experimental groups incubated in the media containing 10% follicular fluid. Smears were prepared after 20 min, 180 min, 24 hours and no incubation times. Sperm chromatin changes like protamine, histone, DNA denaturation, sperm chromatin stability and motility were evaluated at different times.
Incubation of sperm in the follicular fluid increased sperms with normal histone, normal chromatin protamine and sperm with normal head size (p < 0.05).
Administration of follicular fluid into the culture media of the sperms that had been separated by swim up method could improve the sperm quality. Further studies are recommended for understanding the mechanism of the structural change of the sperm chromatin.
卵泡液（FF）是一种富含营养物质、生长因子和激素的生物液体，可能会影响精子质量。到目前为止，精子洗涤一直是在实验室程序中使用常规培养基进行的。
本研究旨在探讨 FF 对精子游出后存活和染色质完整性维持的影响。
将每个洗涤后的精液样本分为两部分；对照组孵育在培养基中，实验组孵育在含有 10%卵泡液的培养基中。在 20 分钟、180 分钟、24 小时和无孵育时间后制备涂片。在不同时间评估精子染色质变化，如鱼精蛋白、组蛋白、DNA 变性、精子染色质稳定性和运动性。
将精子在卵泡液中孵育可增加具有正常组蛋白、正常染色质鱼精蛋白和正常头部大小的精子（p < 0.05）。
将卵泡液添加到已通过游出法分离的精子的培养基中，可以提高精子质量。建议进一步研究以了解精子染色质结构变化的机制。
