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不同培养基对密度梯度离心/上游法和上游法临床结果的影响比较

Comparison of the effect of different media on the clinical outcomes of the density-gradient centrifugation/swim-up and swim-up methods.

作者信息

Kim Eun-Kyung, Kim Eun-Ha, Kim Eun-Ah, Lee Kyung-Ah, Shin Ji-Eun, Kwon Hwang

机构信息

Fertility Center, CHA Bundang Medical Center, Seongnam, Korea.

Department of Biomedical Science, College of Life Science, Seongnam, Korea.

出版信息

Clin Exp Reprod Med. 2015 Mar;42(1):22-9. doi: 10.5653/cerm.2015.42.1.22. Epub 2015 Mar 31.

DOI:10.5653/cerm.2015.42.1.22
PMID:25874170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4390677/
Abstract

OBJECTIVE

Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and have appropriate developmental abilities. The objective of this study was to determine the most optimal sperm preparation method for IVF.

METHODS

Patients less than 40 years of age who participated in a fresh IVF-ET cycle from November 2012 to March 2013 were included in this study. Poor responders with less than three mature oocytes were excluded. Ham's F-10 medium or sperm-washing medium (SWM) was used in combination with the density-gradient centrifugation/swim-up (DGC-SUP) or SUP methods for sperm preparation. A total of 429 fresh IVF-ET cycles were grouped according to the media and methods used for sperm preparation and retrospectively analyzed (DGC-SUP/Ham's F-10, n=82; DGC-SUP/SWM, n=43; SUP/Ham's F-10, n=181; SUP/SWM, n=123).

RESULTS

There were no significant differences among these four groups with respect to the mean age of the female partners, duration of infertility, number of previous IVF cycles, and retrieved oocytes. We determined that both the DGC-SUP and SUP methods for sperm preparation from whole semen, using either Ham's F-10 or SWM media, result in comparable clinical outcomes, including fertilization and pregnancy rates.

CONCLUSION

We suggest that both media and both methods for sperm preparation can be used for selecting high-quality sperm for assistive reproductive technology programs.

摘要

目的

在体外受精-胚胎移植（IVF-ET）程序中，必须对精子进行适当处理，以控制受精率，并确保胚胎质量高且具有适当的发育能力。本研究的目的是确定IVF中最优化的精子处理方法。

方法

本研究纳入了2012年11月至2013年3月期间参与新鲜IVF-ET周期的年龄小于40岁的患者。排除成熟卵母细胞少于3个的低反应者。采用哈姆氏F-10培养基或精子洗涤培养基（SWM），结合密度梯度离心/上游法（DGC-SUP）或上游法（SUP）进行精子处理。根据用于精子处理的培养基和方法，将总共429个新鲜IVF-ET周期分组并进行回顾性分析（DGC-SUP/哈姆氏F-10，n = 82；DGC-SUP/SWM，n = 43；SUP/哈姆氏F-10，n = 181；SUP/SWM，n = 123）。

结果

这四组在女性伴侣的平均年龄、不孕持续时间、既往IVF周期数和回收的卵母细胞数量方面无显著差异。我们确定，使用哈姆氏F-10或SWM培养基从全精液中制备精子的DGC-SUP和SUP方法，在包括受精率和妊娠率在内的临床结局方面相当。

结论

我们建议，两种培养基和两种精子处理方法均可用于辅助生殖技术程序中选择高质量精子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/618d/4390677/509756c6ad5e/cerm-42-22-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/618d/4390677/509756c6ad5e/cerm-42-22-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/618d/4390677/509756c6ad5e/cerm-42-22-g001.jpg

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J Assist Reprod Genet. 2014 Sep;31(9):1139-45. doi: 10.1007/s10815-014-0274-4. Epub 2014 Jun 11.
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