Chemical Science Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
PLoS One. 2012;7(11):e49138. doi: 10.1371/journal.pone.0049138. Epub 2012 Nov 28.
Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn's disease and aids in identification of novel diagnostic targets and disease specific biomarkers.
克罗恩病（CD）是一种病因复杂的炎症性肠病，尽管肠道微生物组的失调与 CD 相关的慢性免疫介导炎症有关。在这里，我们结合了 shotgun 宏基因组学和宏蛋白质组学方法，以鉴定来自六对双胞胎的粪便样本中与 CD 相关的潜在功能特征，这些双胞胎要么健康，要么在回肠（ICD）或结肠（CCD）患有 CD。这些组学方法的整合揭示了几个主要区分 ICD 与健康受试者的基因、蛋白质和途径，包括 ICD 中许多蛋白质的耗竭。此外，ICD 表型与细菌碳水化合物代谢、细菌-宿主相互作用以及人类宿主分泌的酶的改变有关。这种生态系统生物学方法强调了肠道微生物群与克罗恩病病理生理学中功能改变之间的联系，并有助于鉴定新的诊断靶点和疾病特异性生物标志物。
