College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, China.
FEMS Microbiol Lett. 2013 Feb;339(1):30-8. doi: 10.1111/1574-6968.12050. Epub 2012 Dec 4.
The aim of this study was to evaluate the probiotic effects of Lactobacillus strains against Vibrio parahaemolyticus causing gastroenteritis. Six-week-old ICR mice were pretreated with four Lactobacillus strains at three dosages, and then challenged with V. parahaemolyticus TGqx01 (serotype O3:K6). The results showed that V. parahaemolyticus TGqx01 caused severe intestinal fluid accumulation (FA) and villi damage in control mice which were pretreated with phosphate-buffered saline. In contrast, significant alleviation of FA was seen in mice pretreated by with a high dose of Lactobacillus strains (P < 0.05, n = 6) but not in mice that received low-dose pretreatments. Among middle-dose treatments, two highly adhesive strains, Lactobacillus rhamnosus H15 and Lactobacillus brevis Y29-4, significantly decreased intestinal FA and villi damage in treated mice (P < 0.05). Two low-adhesive strains, Lactobacillus acidophilus Y14-3 and Lactobacillus fermentum F16-6, had no significant alleviating effects. At the same dosing levels, no significant differences in FA were observed in mice pretreated with strains with similar adhesive abilities but different antagonistic activities. Our findings suggest that Lactobacillus strains can alleviate V. parahaemolyticus-induced intestinal FA in mice, and the doses required for in vivo efficacy depend more on adhesive ability than on the antibacterial activity of strains.
本研究旨在评估乳酸杆菌菌株对引起肠胃炎的副溶血性弧菌的益生菌作用。将 6 周龄 ICR 小鼠用四种乳酸杆菌菌株在三种剂量下预处理，然后用副溶血性弧菌 TGqx01（血清型 O3:K6）进行攻毒。结果表明，用磷酸盐缓冲盐水预处理的对照组小鼠中，副溶血性弧菌 TGqx01 引起严重的肠道液体积聚（FA）和绒毛损伤。相比之下，高剂量乳酸杆菌菌株预处理的小鼠中明显减轻了 FA（P<0.05，n=6），但低剂量预处理的小鼠没有。在中剂量处理中，两种高粘附性菌株，鼠李糖乳杆菌 H15 和短乳杆菌 Y29-4，显著减轻了治疗小鼠的肠道 FA 和绒毛损伤（P<0.05）。两种低粘附性菌株，嗜酸乳杆菌 Y14-3 和发酵乳杆菌 F16-6，没有明显的缓解作用。在相同的剂量水平下，用具有相似粘附能力但拮抗活性不同的菌株预处理的小鼠中，FA 没有显著差异。我们的研究结果表明，乳酸杆菌菌株可以减轻副溶血性弧菌诱导的小鼠肠道 FA，体内疗效所需的剂量更多地取决于菌株的粘附能力而不是抗菌活性。
