Department of Pharmaceutical Biochemistry, Medical University of Gdansk, Gdansk, Poland.
J Endocrinol Invest. 2013 Jun;36(6):428-34. doi: 10.3275/8770. Epub 2012 Nov 27.
Chemerin is a newly discovered adipokine, whose circulating concentration is increased in obesity.
To elucidate whether the increased circulating chemerin concentrations in obese subjects are associated with the increase of fat mass, the increase in chemerin gene expression in adipose tissue or both.
MATERIAL/SUBJECTS AND METHODS: Serum chemerin concentrations in 20 non-obese healthy volunteers and 21 non-diabetic obese subjects were measured using ELISA. Chemerin mRNA and chemerin protein levels in visceral and subcutaneous adipose tissues of obese subjects were analyzed by Real-Time PCR and Western blot respectively.
We found that the serum chemerin concentrations were significantly higher in obese subjects than in controls and positively correlated with BMI, fat mass and body mass. Moreover serum chemerin concentrations were correlated positively with serum CRP concentrations independently of BMI. No correlation was found between the chemerin mRNA and chemerin protein levels in visceral and subcutaneous adipose tissues and BMI, fat mass, or body weight. Likewise, there was no correlation between the serum chemerin concentrations and the levels of chemerin mRNA and protein in adipose tissue of obese patients. Multiple regression analysis suggests that BMI was the main predictor of serum chemerin concentration. In contrast to chemerin, both serum leptin concentrations and adipose tissue leptin mRNA levels positively correlated with BMI.
The results presented here indicate that serum chemerin concentrations correlated with BMI, whereas chemerin mRNA levels in adipose tissue did not. Thus the elevated circulating chemerin concentration in obese, non-diabetic patients was mainly associated with the increased BMI.
趋化素是一种新发现的脂肪因子，其循环浓度在肥胖症中增加。
阐明肥胖患者循环趋化素浓度的增加是与脂肪量的增加有关，还是与脂肪组织中趋化素基因表达的增加有关，或者两者都有关。
材料/研究对象和方法：使用 ELISA 法测量 20 名非肥胖健康志愿者和 21 名非糖尿病肥胖患者的血清趋化素浓度。通过实时 PCR 和 Western blot 分别分析肥胖患者内脏和皮下脂肪组织中的趋化素 mRNA 和趋化素蛋白水平。
我们发现，肥胖患者的血清趋化素浓度明显高于对照组，且与 BMI、脂肪量和体重呈正相关。此外，血清趋化素浓度与 BMI 独立相关，与血清 CRP 浓度呈正相关。内脏和皮下脂肪组织中的趋化素 mRNA 和趋化素蛋白水平与 BMI、脂肪量或体重之间均无相关性。同样，肥胖患者的血清趋化素浓度与脂肪组织中的趋化素 mRNA 和蛋白水平之间也无相关性。多元回归分析表明，BMI 是血清趋化素浓度的主要预测因子。与趋化素相反，血清瘦素浓度和脂肪组织瘦素 mRNA 水平与 BMI 呈正相关。
本研究结果表明，血清趋化素浓度与 BMI 相关，而脂肪组织中的趋化素 mRNA 水平则不相关。因此，肥胖、非糖尿病患者循环中趋化素浓度的升高主要与 BMI 的增加有关。
