Li Xue-Mei, Ji Hui, Li Chun-Jun, Wang Peng-Hua, Yu Pei, Yu De-Min
The Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.
Department of obstetrics, Tianjin Central gynaecology and obstetrics Hospital, Tianjin, China.
Ann Endocrinol (Paris). 2015 Feb;76(1):19-24. doi: 10.1016/j.ando.2014.10.001. Epub 2014 Nov 10.
The aim of this study was to determine the effect of obesity, gestational diabetes mellitus (GDM) on circulating chemerin concentrations and chemerin gene expression of adipotissue in pregnancy women.
Totally 42 normal glucose tolerant (NGT) women and 48 women with GDM were included in this study. Their clinical features and biochemical parameters were analyzed. The NGT and GDM women were subgrouped by prepregnancy BMI as normal-weight group, overweight group, and obese group, respectively. Serum chemerin and tumor necrosis factor α (TNF-α) of these individuals were determined by ELISA methods, and subcutaneous adipose tissues' mRNA expressions of chemerin and CMKLR1 (encoding the receptor of chemerin) were analyzed by real-time PCR.
Serum chemerin in obese-NGT group and normal-weight-GDM group was significantly higher than that of normal-weight-NGT group. Chemerin and CMKLR1 mRNA expression of subcutaneous adipose tissue was lower in the obese-NGT group than normal-weight-NGT group. There was no significant difference of CMKLR1 mRNA expression between normal-weight-NGT and normal-weight-GDM group. Serum chemerin significantly and positively correlated with triglycerides (TG) and homeostasis model assessment of insulin resistance (HOMA-IR) assessed both by uni- and multivariate.
Gestational obesity, GDM may contribute to the elevating serum chemerin. Serum chemerin in pregnancy was associated with insulin resistance and triglycerides. Chemerin gene may play a role both in obese and GDM patients. CMKLR1 might exert its action only in obese individuals, not in GDM patients before delivery.
本研究旨在确定肥胖、妊娠期糖尿病（GDM）对孕妇循环中chemerin浓度及脂肪组织中chemerin基因表达的影响。
本研究共纳入42例糖耐量正常（NGT）女性和48例GDM女性。分析她们的临床特征和生化参数。NGT和GDM女性按孕前体重指数分别分为正常体重组、超重组和肥胖组。采用ELISA法测定这些个体的血清chemerin和肿瘤坏死因子α（TNF-α），并通过实时PCR分析皮下脂肪组织中chemerin和CMKLR1（编码chemerin受体）的mRNA表达。
肥胖-NGT组和正常体重-GDM组的血清chemerin显著高于正常体重-NGT组。肥胖-NGT组皮下脂肪组织中chemerin和CMKLR1 mRNA表达低于正常体重-NGT组。正常体重-NGT组和正常体重-GDM组之间CMKLR1 mRNA表达无显著差异。血清chemerin与甘油三酯（TG）和胰岛素抵抗稳态模型评估（HOMA-IR）在单变量和多变量分析中均显著正相关。
妊娠期肥胖、GDM可能导致血清chemerin升高。孕期血清chemerin与胰岛素抵抗和甘油三酯有关。chemerin基因可能在肥胖和GDM患者中均起作用。CMKLR1可能仅在肥胖个体中发挥作用，而在分娩前的GDM患者中不发挥作用。
