Bhadaniya Amit R, Kalariya Vinay A, Joshi Dilip V, Patel Bakor J, Chaudhary Sandhya, Patel Hitesh B, Patel Jignesh M, Patel Urvesh D, Patel Harshad B, Ghodasara Sanjay N, Savsani Harish H
Department of Veterinary Pathology, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India
Department of Veterinary Pathology, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India.
Toxicol Ind Health. 2015 Jan;31(1):9-17. doi: 10.1177/0748233712468017. Epub 2012 Dec 4.
The present study was carried out to evaluate the effects of exposure at different doses of acephate on hematology, blood biochemistry, oxidative stress and immune system of Wistar rats. The experiment was carried out on 40 Wistar rats, which were divided in four groups. Animals of the three treatment groups were given with different sublethal doses (1/40th, 1/20th, 1/10th of lethal dose 50 value) of acephate by oral gavage. The hematology, blood biochemistry, oxidative stress marker, humoral immune response and cell-mediated immunity were evaluated following acephate exposure. Significant alteration in hematological parameters was not observed following different doses of acephate; however, significant alteration in alkaline phosphatase, gamma glutamyl transferase, acetyl cholinesterase, lipid peroxidase and superoxide dismutase was observed in medium- and high-dose group animals. Nonsignificant decrease in antibody titer in animals exposed to high dose has been observed compared with animals of control group. However, significant alteration in cell-mediated immunity was not observed in animals treated with acephate at different doses.
本研究旨在评估不同剂量乙酰甲胺磷暴露对Wistar大鼠血液学、血液生化、氧化应激和免疫系统的影响。实验选用40只Wistar大鼠，分为四组。三个处理组的动物通过灌胃给予不同的亚致死剂量（半数致死量的1/40、1/20、1/10）的乙酰甲胺磷。在乙酰甲胺磷暴露后，对血液学、血液生化、氧化应激标志物、体液免疫反应和细胞介导免疫进行评估。不同剂量的乙酰甲胺磷暴露后，血液学参数未观察到显著变化；然而，在中、高剂量组动物中观察到碱性磷酸酶、γ-谷氨酰转移酶、乙酰胆碱酯酶、脂质过氧化物酶和超氧化物歧化酶有显著变化。与对照组动物相比，高剂量暴露动物的抗体滴度有不显著下降。然而，不同剂量乙酰甲胺磷处理的动物未观察到细胞介导免疫有显著变化。
