Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Rodovia Celso Garcia Cid, Pr 445 Km 380, Londrina, Paraná, 86057-970, Brazil.
Department of Biotechnology, Genetics and Cell Biology, State University of Maringá - UEM, 5790, Maringá, Paraná, 87020-900, Brazil.
Environ Sci Pollut Res Int. 2020 Feb;27(5):5482-5488. doi: 10.1007/s11356-019-07209-0. Epub 2019 Dec 18.
Acephate is an organophosphate insecticide that disrupts the endocrine system and impairs the male reproductive system. Thus, the aim of the present study was to evaluate whether exposure to acephate during maternal gestation and lactation histologically damages the testes of male Wistar rats in adulthood. For this study, adult Wistar rats were divided into the following groups: ACE-mother, (2.5 mg/kg/bw, gestational day (GD) 7 to postnatal day (PND) 21) and oil-mother (corn oil (control), GD 7 to PND 21). The male offspring (PND 90) were euthanized, and the prostates and testes were collected and weighed. The testes were utilized for histopathological analyses and to determine the sperm count. A spermatogenesis kinetic analysis revealed an increased number of seminiferous tubules in stages I-VI in the ACE-mother group. Additionally, we observed a decrease in the epithelium and the diameters of the evaluated seminiferous tubules and in the number of Sertoli cells in the group exposed to acephate. The sperm count analysis showed no difference between the groups. We conclude that maternal exposure to the pesticide acephate did not affect testicular function, but led to the impairment of testicular development and morphology of the tissue in adulthood.
乙酰甲胺磷是一种有机磷杀虫剂，会破坏内分泌系统并损害男性生殖系统。因此，本研究旨在评估母体妊娠和哺乳期接触乙酰甲胺磷是否会在成年雄性 Wistar 大鼠的睾丸组织中造成组织学损伤。为此，将成年 Wistar 大鼠分为以下几组：ACE-母亲组（2.5mg/kg/bw，妊娠第 7 天至产后第 21 天）和油-母亲组（玉米油（对照），妊娠第 7 天至产后第 21 天）。雄性幼鼠（PND90）被安乐死，收集前列腺和睾丸并称重。对睾丸进行组织病理学分析和精子计数。精子发生动力学分析显示，ACE-母亲组的 I-VI 期生精小管数量增加。此外，我们观察到暴露于乙酰甲胺磷的组中上皮和评估的生精小管的直径以及支持细胞的数量减少。精子计数分析显示两组之间没有差异。我们得出结论，母体接触农药乙酰甲胺磷不会影响睾丸功能，但会导致成年后睾丸发育和组织形态受损。
