微小 RNA-205 直接调控人 KB 口腔癌细胞中的肿瘤抑制因子白细胞介素-24。
MicroRNA-205 directly regulates the tumor suppressor, interleukin-24, in human KB oral cancer cells.
机构信息
Oral Biology Research Institute, Chosun University School of Dentistry, Gwangju, Korea.
出版信息
Mol Cells. 2013 Jan;35(1):17-24. doi: 10.1007/s10059-013-2154-7. Epub 2012 Dec 3.
Abstract
MicroRNA (miRNA) is a form of small noncoding RNA that regulates the expression of genes either by inhibiting mRNA translation or by inducing its degradation. Small microRNA play important roles in regulating a large number of cellular processes, including development, proliferation and apoptosis. This study examined the biological functions of miR-205 as a tumor suppressor in KB oral cancer cells. The results showed that miR-205 expression was significantly lower in KB oral cancer cells than in human normal oral keratinocytes. Furthermore, the miR-205 over-expressed in KB oral cancer cells increased the cell cytotoxicity and induced apoptosis through the activation of caspase-3/-7. The transfection of miR-205 into KB oral cancer cells strongly induced IL-24, a well known cytokine that acts as a tumor suppressor in a range of tumor tissues. In addition, miR-205 targeted the IL-24 promoter directly to induce gene expression. Overall, miR-205 has significant therapeutic potential to turn on silenced tumor suppressor genes by targeting them with miRNA.
摘要
微小 RNA（miRNA）是一种小型非编码 RNA，通过抑制 mRNA 翻译或诱导其降解来调节基因的表达。小 miRNA 在调节许多细胞过程中发挥着重要作用，包括发育、增殖和凋亡。本研究探讨了 miR-205 作为肿瘤抑制因子在 KB 口腔癌细胞中的生物学功能。结果表明，miR-205 在 KB 口腔癌细胞中的表达明显低于人正常口腔角质形成细胞。此外，在 KB 口腔癌细胞中过表达 miR-205 通过激活 caspase-3/-7 增加细胞细胞毒性并诱导细胞凋亡。miR-205 转染到 KB 口腔癌细胞中强烈诱导 IL-24，IL-24 是一种在多种肿瘤组织中作为肿瘤抑制因子发挥作用的已知细胞因子。此外，miR-205 直接靶向 IL-24 启动子诱导基因表达。总的来说，miR-205 通过用 miRNA 靶向沉默的肿瘤抑制基因，具有显著的治疗潜力。
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