高效液相色谱法同时测定化妆品中四种呋喃香豆素

[Simultaneous determination of four kinds of furocoumarins in cosmetics by high performance liquid chromatography].

作者信息

Liu Baojun, Yan Lili, Li Qiuyue, Wang Jinfang, Shi Yue

机构信息

State Food and Drug Administration Review of Health Food Center, Beijing 100070, China.

出版信息

Wei Sheng Yan Jiu. 2012 Sep;41(5):790-3.

PMID:23213695

Abstract

OBJECTIVE

A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of 4 furocoumarins, including 8-methoxycoumarin, 5-methoxycoumarin, trioxsalen and imperatorin in cosmetics.

METHODS

The separation was carried on Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) by gradient elution with methanol-water as mobile phase at a flow rate of 1.0 ml/min and column temperature of 30 degrees C. The analysis of 4 furocoumarins was performed by HPLC with diodearray detector at the wavelength of 248 nm.

RESULTS

Identification of 4 furocoumarins was achieved by retention time, and quantification analysis was performed by the external standard method. The limits of quantitation (LOQ) for 4 furocoumarins were 0.54, 0.52, 0.58 and 0.50 microg/g, respectively. The method showed a good linearity in the range of 0.05-10 microg/ml with correlation coefficients more than 0.999. The mean recoveries at spiked levels were in the range of 89.9%-105.2% with RSDs of 0.20%-1.08%.

CONCLUSION

The method was accurate and simple, and was suitable for the determination of 4 furocoumarins in cosmetics.

摘要

目的

建立一种高效液相色谱（HPLC）法，用于同时测定化妆品中的4种呋喃香豆素，包括8-甲氧基香豆素、5-甲氧基香豆素、三甲沙林和欧前胡素。

方法

采用Diamonsil C18柱（4.6 mm×250 mm，5 µm），以甲醇-水为流动相进行梯度洗脱，流速为1.0 ml/min，柱温为30℃。采用二极管阵列检测器，在波长248 nm处对4种呋喃香豆素进行HPLC分析。

结果

通过保留时间对4种呋喃香豆素进行鉴定，采用外标法进行定量分析。4种呋喃香豆素的定量限（LOQ）分别为0.54、0.52、0.58和0.50 μg/g。该方法在0.05-10 μg/ml范围内线性良好，相关系数大于0.999。加标水平下的平均回收率在89.9%-105.2%之间，相对标准偏差（RSD）为0.20%-1.08%。